| Literature DB >> 12447372 |
Pamela M Pollock1, Ursula L Harper, Katherine S Hansen, Laura M Yudt, Mitchell Stark, Christiane M Robbins, Tracy Y Moses, Galen Hostetter, Urs Wagner, John Kakareka, Ghadi Salem, Tom Pohida, Peter Heenan, Paul Duray, Olli Kallioniemi, Nicholas K Hayward, Jeffrey M Trent, Paul S Meltzer.
Abstract
To evaluate the timing of mutations in BRAF (v-raf murine sarcoma viral oncogene homolog B1) during melanocytic neoplasia, we carried out mutation analysis on microdissected melanoma and nevi samples. We observed mutations resulting in the V599E amino-acid substitution in 41 of 60 (68%) melanoma metastases, 4 of 5 (80%) primary melanomas and, unexpectedly, in 63 of 77 (82%) nevi. These data suggest that mutational activation of the RAS/RAF/MAPK pathway in nevi is a critical step in the initiation of melanocytic neoplasia but alone is insufficient for melanoma tumorigenesis.Entities:
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Year: 2002 PMID： 12447372 DOI： 10.1038/ng1054
Source DB: PubMed Journal: Nat Genet ISSN： 1061-4036 Impact factor: 38.330