| Literature DB >> 12445654 |
Caroline Choma1, Per Einar Granum.
Abstract
A polymerase chain reaction (PCR) fragment from strain NVH 38 (containing bceT) was cloned, sequenced and expressed in Escherichia coli. This sequence showed 50-60% identity to the original. When this bceT clone was expressed in E. coli no biological activity was found in either supernatants or cell extracts. Cell extracts from the Bacillus cereus strains (NVH 38 and B-4ac) were also negative on Vero cells. Neutralisation of supernatant from B. cereus B-4ac using a monoclonal antibody (reacting with NheB and HblD) abolished the activity. A test for cytotoxic enterotoxins was negative for both cell extracts and supernatants. Our data suggest that BcET either has an unknown type enterotoxic action or non at all.Entities:
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Year: 2002 PMID: 12445654 DOI: 10.1111/j.1574-6968.2002.tb11464.x
Source DB: PubMed Journal: FEMS Microbiol Lett ISSN: 0378-1097 Impact factor: 2.742