Literature DB >> 12445654

The enterotoxin T (BcET) from Bacillus cereus can probably not contribute to food poisoning.

Caroline Choma1, Per Einar Granum.   

Abstract

A polymerase chain reaction (PCR) fragment from strain NVH 38 (containing bceT) was cloned, sequenced and expressed in Escherichia coli. This sequence showed 50-60% identity to the original. When this bceT clone was expressed in E. coli no biological activity was found in either supernatants or cell extracts. Cell extracts from the Bacillus cereus strains (NVH 38 and B-4ac) were also negative on Vero cells. Neutralisation of supernatant from B. cereus B-4ac using a monoclonal antibody (reacting with NheB and HblD) abolished the activity. A test for cytotoxic enterotoxins was negative for both cell extracts and supernatants. Our data suggest that BcET either has an unknown type enterotoxic action or non at all.

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Year:  2002        PMID: 12445654     DOI: 10.1111/j.1574-6968.2002.tb11464.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN: 0378-1097            Impact factor:   2.742


  5 in total

1.  Distribution of genes encoding putative virulence factors and fragment length polymorphisms in the vrrA gene among Brazilian isolates of Bacillus cereus and Bacillus thuringiensis.

Authors:  Viviane Zahner; Diana Aparecida Cabral; Adriana Hamond Régua-Mangia; Leon Rabinovitch; Gaétan Moreau; Douglas McIntosh
Journal:  Appl Environ Microbiol       Date:  2005-12       Impact factor: 4.792

2.  Rapid Ped-2E9 cell-based cytotoxicity analysis and genotyping of Bacillus species.

Authors:  Kristen M Gray; Padmapriya P Banada; Erin O'Neal; Arun K Bhunia
Journal:  J Clin Microbiol       Date:  2005-12       Impact factor: 5.948

Review 3.  Detection and Enumeration of Spore-Forming Bacteria in Powdered Dairy Products.

Authors:  Aoife J McHugh; Conor Feehily; Colin Hill; Paul D Cotter
Journal:  Front Microbiol       Date:  2017-01-31       Impact factor: 5.640

4.  Toxigenic potential and antimicrobial susceptibility of Bacillus cereus group bacteria isolated from Tunisian foodstuffs.

Authors:  Maroua Gdoura-Ben Amor; Sophie Jan; Florence Baron; Noël Grosset; Antoine Culot; Radhouane Gdoura; Michel Gautier; Clarisse Techer
Journal:  BMC Microbiol       Date:  2019-08-24       Impact factor: 3.605

Review 5.  The Food Poisoning Toxins of Bacillus cereus.

Authors:  Richard Dietrich; Nadja Jessberger; Monika Ehling-Schulz; Erwin Märtlbauer; Per Einar Granum
Journal:  Toxins (Basel)       Date:  2021-01-28       Impact factor: 4.546

  5 in total

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