Literature DB >> 12441644

Virus-induced silencing of FtsH gene in Nicotiana benthmiana causes a striking bleached leaf phenotype.

Hiromasa Saitoh1, Ryohei Terauchi.   

Abstract

A recombinant Potato virus X (PVX) vector, pTXS.FtsH, harboring partial sequence of FtsH gene of Nicotiana benthamiana was constructed to silence the expression of endogenous FtsH homologous gene in N. benthamiana. Inoculation with in vitro runoff transcript of pTXS.FtsH to N. benthamiana plants allowed silencing of FtsH, causing striking bleaching of upper leaves reminiscent of var2 mutant phenotype of Arabidopsis thaliana. FtsH-silenced plants exhibited no resistance against Tobacco mosaic virus (TMV) and a phytopathogenic fungus Botrytis cinerea. Virus-induced gene silencing (VIGS) of N. benthamiana with PVX as demonstrated here would be an efficient and rapid method to study the function of other elements of photosystem II (PSII) in planta.

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Year:  2002        PMID： 12441644     DOI： 10.1266/ggs.77.335

Source DB:  PubMed          Journal:  Genes Genet Syst        ISSN： 1341-7568            Impact factor:   1.517


  6 in total

1.  Chilli leaf curl virus infection downregulates the expression of the genes encoding chloroplast proteins and stress-related proteins.

Authors:  Nirbhay Kumar Kushwaha; Pranav Pankaj Sahu; Manoj Prasad; Supriya Chakrabroty
Journal:  Physiol Mol Biol Plants       Date:  2019-08-22

Review 2.  Principles and practice of virus induced gene silencing for functional genomics in plants.

Authors:  Neelam Jagram; Indranil Dasgupta
Journal:  Virus Genes       Date:  2022-10-20       Impact factor: 2.198

3.  Virus-induced silencing of WIPK and SIPK genes reduces resistance to a bacterial pathogen, but has no effect on the INF1-induced hypersensitive response (HR) in Nicotiana benthamiana.

Authors:  P C Sharma; A Ito; T Shimizu; R Terauchi; S Kamoun; H Saitoh
Journal:  Mol Genet Genomics       Date:  2003-07-01       Impact factor: 3.291

4.  Serial Analysis of Gene Expression (SAGE) of Magnaporthe grisea: genes involved in appressorium formation.

Authors:  T Irie; H Matsumura; R Terauchi; H Saitoh
Journal:  Mol Genet Genomics       Date:  2003-08-29       Impact factor: 3.291

5.  Assessing the tobacco-rattle-virus-based vectors system as an efficient gene silencing technique in Datura stramonium (Solanaceae).

Authors:  Mohammad Reza Eftekhariyan Ghamsari; Farah Karimi; Seyed Latif Mousavi Gargari; Seyed Ali Hosseini Tafreshi; Seyed Alireza Salami
Journal:  Virus Genes       Date:  2014-07-29       Impact factor: 2.332

6.  Virus-induced gene silencing of 14-3-3 genes abrogates dark repression of nitrate reductase activity in Nicotiana benthamiana.

Authors:  Tatsuya Hirano; Akiko Ito; Thomas Berberich; Ryohei Terauchi; Hiromasa Saitoh
Journal:  Mol Genet Genomics       Date:  2007-04-19       Impact factor: 2.980
  6 in total

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