BACKGROUND: Glutathione S-transferases (GST) are a family of polymorphic enzymes responsible for the metabolism of a broad range of xenobiotics including carcinogens and endogenous compounds. METHODS: The aim of this study was to develop a fully automated spectrophotometric method to determine serum GST activity. RESULTS: The method had a detection limit of 7 U/l. The within- and between-run repeatability reached 2% and 3%, respectively. The reproducibility tested over a 6-month period was 7%. Serum GST activity is stable for at least 1 week at +37, +4 and -20 degrees C, 1 month at -20 degrees C and 6 months at -80 and -196 degrees C. The GST activities were determined on 374 supposedly healthy subjects coming to the Center of Preventive Medicine for a health screening. The sample population comprised 178 males and 196 females 4-80 years of age. The mean value of the serum GST activity calculated for this healthy population was 28.2+/-3.0 U/l without gender difference (range from 20.0 to 38.0 U/l). The global interindividual variability reached 11%. CONCLUSIONS: This report describes a spectrophotometric assay for monitoring the serum GST activities in human. This method will be useful in examining the relationships between serum GST activities and the GST polymorphisms, especially pi-GST.
BACKGROUND: Glutathione S-transferases (GST) are a family of polymorphic enzymes responsible for the metabolism of a broad range of xenobiotics including carcinogens and endogenous compounds. METHODS: The aim of this study was to develop a fully automated spectrophotometric method to determine serum GST activity. RESULTS: The method had a detection limit of 7 U/l. The within- and between-run repeatability reached 2% and 3%, respectively. The reproducibility tested over a 6-month period was 7%. Serum GST activity is stable for at least 1 week at +37, +4 and -20 degrees C, 1 month at -20 degrees C and 6 months at -80 and -196 degrees C. The GST activities were determined on 374 supposedly healthy subjects coming to the Center of Preventive Medicine for a health screening. The sample population comprised 178 males and 196 females 4-80 years of age. The mean value of the serum GST activity calculated for this healthy population was 28.2+/-3.0 U/l without gender difference (range from 20.0 to 38.0 U/l). The global interindividual variability reached 11%. CONCLUSIONS: This report describes a spectrophotometric assay for monitoring the serum GST activities in human. This method will be useful in examining the relationships between serum GST activities and the GST polymorphisms, especially pi-GST.
Authors: Martin Laschak; Klaus-Dieter Spindler; Andres J Schrader; Andrea Hessenauer; Wolfgang Streicher; Mark Schrader; Marcus V Cronauer Journal: BMC Cancer Date: 2012-03-30 Impact factor: 4.430
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Authors: Leandro V B Carvalho; Sandra S Hacon; Claudia M Vega; Jucilene A Vieira; Ariane L Larentis; Rita C O C Mattos; Daniel Valente; Isabele C Costa-Amaral; Dennys S Mourão; Gabriela P Silva; Beatriz F A Oliveira Journal: Int J Environ Res Public Health Date: 2019-07-27 Impact factor: 3.390
Authors: Rafael C Lajmanovich; Paola M Peltzer; Andrés M Attademo; Candela S Martinuzzi; María F Simoniello; Carlina L Colussi; Ana P Cuzziol Boccioni; Mirna Sigrist Journal: Heliyon Date: 2019-10-25