Literature DB >> 12409428

Quantification of hepatitis C virus in human liver and serum samples by using LightCycler reverse transcriptase PCR.

Peter A White1, Yong Pan, Anthony J Freeman, George Marinos, Rosemary A Ffrench, Andrew R Lloyd, William D Rawlinson.   

Abstract

A highly sensitive, non-probe-based, real-time quantitative reverse transcriptase PCR was developed for viral load measurements in both serum and liver samples from patients with hepatitis C virus (HCV) infection. With synthetic RNA, the linearity of the approach was conserved over a wide range of HCV copy numbers. There was a strong correlation between hepatic and serum viral load measurements (r = 0.689, P = 0.004, n = 15), indicating that the level of viremia reflected the amount of virus present in the liver.

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Year:  2002        PMID： 12409428      PMCID： PMC139708          DOI： 10.1128/JCM.40.11.4346-4348.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  22 in total

1.  Hepatitis C virus: quantitation and distribution in liver.

Authors:  N A Terrault; P J Dailey; L Ferrell; M L Collins; J C Wilber; M S Urdea; B N Bhandari; T L Wright
Journal:  J Med Virol       Date:  1997-03       Impact factor: 2.327

2.  Quantification of the initial decline of serum hepatitis C virus RNA and response to interferon alfa.

Authors:  S Zeuzem; J H Lee; A Franke; B Rüster; O Prümmer; G Herrmann; W K Roth
Journal:  Hepatology       Date:  1998-04       Impact factor: 17.425

3.  Real-time detection system for quantitation of hepatitis C virus RNA: a comparison with the other three methods.

Authors: 
Journal:  Hepatol Res       Date:  2001-01-01       Impact factor: 4.288

4.  Evaluation of a new enzyme immunoassay for hepatitis C virus (HCV) core antigen with clinical sensitivity approximating that of genomic amplification of HCV RNA.

Authors:  E Tanaka; C Ohue; K Aoyagi; K Yamaguchi; S Yagi; K Kiyosawa; H J Alter
Journal:  Hepatology       Date:  2000-08       Impact factor: 17.425

5.  Estimating progression to cirrhosis in chronic hepatitis C virus infection.

Authors:  A J Freeman; G J Dore; M G Law; M Thorpe; J Von Overbeck; A R Lloyd; G Marinos; J M Kaldor
Journal:  Hepatology       Date:  2001-10       Impact factor: 17.425

6.  Intrahepatic hepatitis C RNA levels do not correlate with degree of liver injury in patients with chronic hepatitis C.

Authors:  P H McGuinness; G A Bishop; D M Painter; R Chan; G W McCaughan
Journal:  Hepatology       Date:  1996-04       Impact factor: 17.425

7.  Efficacy of interferon alfa therapy in chronic hepatitis C patients depends primarily on hepatitis C virus RNA level.

Authors:  G Yamada; M Takatani; F Kishi; M Takahashi; T Doi; T Tsuji; S Shin; M Tanno; M S Urdea; J A Kolberg
Journal:  Hepatology       Date:  1995-11       Impact factor: 17.425

8.  Detection of hepatitis C virus RNA by a combined reverse transcription-polymerase chain reaction assay.

Authors:  K K Young; R M Resnick; T W Myers
Journal:  J Clin Microbiol       Date:  1993-04       Impact factor: 5.948

9.  An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis.

Authors:  G Kuo; Q L Choo; H J Alter; G L Gitnick; A G Redeker; R H Purcell; T Miyamura; J L Dienstag; M J Alter; C E Stevens
Journal:  Science       Date:  1989-04-21       Impact factor: 47.728

10.  Simplified hepatitis C virus genotyping by heteroduplex mobility analysis.

Authors:  P A White; X Zhai; I Carter; Y Zhao; W D Rawlinson
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948
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  15 in total

1.  Strong correlation between liver and serum levels of hepatitis C virus core antigen and RNA in chronically infected patients.

Authors:  V Descamps; A Op de Beeck; C Plassart; E Brochot; C François; F Helle; M Adler; N Bourgeois; D Degré; G Duverlie; S Castelain
Journal:  J Clin Microbiol       Date:  2011-12-07       Impact factor: 5.948

Review 2.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

3.  Norovirus excretion in an aged-care setting.

Authors:  Elise T-V Tu; Rowena A Bull; Mi-Jurng Kim; Christopher J McIver; Leon Heron; William D Rawlinson; Peter A White
Journal:  J Clin Microbiol       Date:  2008-04-16       Impact factor: 5.948

4.  Transfer and evaluation of an automated, low-cost real-time reverse transcription-PCR test for diagnosis and monitoring of human immunodeficiency virus type 1 infection in a West African resource-limited setting.

Authors:  Francois Rouet; Didier K Ekouevi; Marie-Laure Chaix; Marianne Burgard; Andre Inwoley; Thomas D'Aquin Tony; Christine Danel; Xavier Anglaret; Valeriane Leroy; Philippe Msellati; Francois Dabis; Christine Rouzioux
Journal:  J Clin Microbiol       Date:  2005-06       Impact factor: 5.948

5.  Hepatic SOCS3 expression is strongly associated with non-response to therapy and race in HCV and HCV/HIV infection.

Authors:  Kyung-Ah Kim; Wenyu Lin; Andrew W Tai; Run-Xuan Shao; Ethan Weinberg; Carolina B De Sa Borges; Atul K Bhan; Hui Zheng; Yoshitaka Kamegaya; Raymond T Chung
Journal:  J Hepatol       Date:  2009-02-14       Impact factor: 25.083

6.  Quantification of hepatitis C virus (HCV) in liver specimens and sera from patients with human immunodeficiency virus coinfection by using the Versant HCV RNA 3.0 (branched DNA-based) DNA assay.

Authors:  Rosamaria Tedeschi; Eliana Pivetta; Stefania Zanussi; Ettore Bidoli; Mirna Ros; Giampiero di Gennaro; Guglielmo Nasti; Paolo De Paoli
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

7.  Dynamics of hepatitis C virus replication in human liver.

Authors:  Ming Chang; Ocean Williams; John Mittler; Adrian Quintanilla; Robert L Carithers; James Perkins; Lawrence Corey; David R Gretch
Journal:  Am J Pathol       Date:  2003-08       Impact factor: 4.307

8.  Use of the MagNA pure LC automated nucleic acid extraction system followed by real-time reverse transcription-PCR for ultrasensitive quantitation of hepatitis C virus RNA.

Authors:  Linda Cook; Ka-Wing Ng; Arthur Bagabag; Lawrence Corey; Keith R Jerome
Journal:  J Clin Microbiol       Date:  2004-09       Impact factor: 5.948

9.  Hepatitis C testing and status among opioid substitution treatment clients in New South Wales.

Authors:  Fiona L Shand; Carolyn Day; William Rawlinson; Louisa Degenhardt; Nicholas G Martin; Elliot C Nelson
Journal:  Aust N Z J Public Health       Date:  2014-04       Impact factor: 2.939

10.  Application of molecular diagnostic techniques for viral testing.

Authors:  Fernando Cobo
Journal:  Open Virol J       Date:  2012-11-30
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