Literature DB >> 16957277

Molecular-beacon multiplex real-time PCR assay for detection of Vibrio cholerae.

Aneta J Gubala1, David F Proll.   

Abstract

A multiplex real-time PCR assay was developed using molecular beacons for the detection of Vibrio cholerae by targeting four important virulence and regulatory genes. The specificity and sensitivity of this assay, when tested with pure culture and spiked environmental water samples, were high, surpassing those of currently published PCR assays for the detection of this organism.

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Year:  2006        PMID： 16957277      PMCID： PMC1563670          DOI： 10.1128/AEM.02597-05

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  27 in total

1.  Development and evaluation of a multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O1 and O139.

Authors:  K Hoshino; S Yamasaki; A K Mukhopadhyay; S Chakraborty; A Basu; S K Bhattacharya; G B Nair; T Shimada; Y Takeda
Journal:  FEMS Immunol Med Microbiol       Date:  1998-03

2.  Detecting and biotyping Vibrio cholerae O1 with multiplex polymerase chain reaction.

Authors:  S P Keasler; R H Hall
Journal:  Lancet       Date:  1993-06-26       Impact factor: 79.321

3.  Rapid detection of Vibrio cholerae O139 Bengal from stool specimens by PCR.

Authors:  M J Albert; D Islam; S Nahar; F Qadri; S Falklind; A Weintraub
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948

4.  Multiplex polymerase chain reaction to detect toxigenic Vibrio cholerae and to biotype Vibrio cholerae O1.

Authors:  Y H Shangkuan; Y S Show; T M Wang
Journal:  J Appl Bacteriol       Date:  1995-09

5.  A gene for the enterotoxin zonula occludens toxin is present in Vibrio mimicus and Vibrio cholerae O139.

Authors:  M A Chowdhury; R T Hill; R R Colwell
Journal:  FEMS Microbiol Lett       Date:  1994-06-15       Impact factor: 2.742

6.  Polymerase chain reaction for detection of the cholera enterotoxin operon of Vibrio cholerae.

Authors:  H Shirai; M Nishibuchi; T Ramamurthy; S K Bhattacharya; S C Pal; Y Takeda
Journal:  J Clin Microbiol       Date:  1991-11       Impact factor: 5.948

7.  Duplex real-time SYBR green PCR assays for detection of 17 species of food- or waterborne pathogens in stools.

Authors:  Hiroshi Fukushima; Yoshie Tsunomori; Ryotaro Seki
Journal:  J Clin Microbiol       Date:  2003-11       Impact factor: 5.948

8.  Reverse transcription-multiplex PCR assay for simultaneous detection of Escherichia coli O157:H7, Vibrio cholerae O1, and Salmonella Typhi.

Authors:  Nicole J Morin; Zhilong Gong; Xing-Fang Li
Journal:  Clin Chem       Date:  2004-09-13       Impact factor: 8.327

9.  Method of DNA extraction and application of multiplex polymerase chain reaction to detect toxigenic Vibrio cholerae O1 and O139 from aquatic ecosystems.

Authors:  Irma N G Rivera; Erin K Lipp; Ana Gil; Nipa Choopun; Anwar Huq; Rita R Colwell
Journal:  Environ Microbiol       Date:  2003-07       Impact factor: 5.491

10.  Use of polymerase chain reaction for detection of toxigenic Vibrio cholerae O1 strains from the Latin American cholera epidemic.

Authors:  P I Fields; T Popovic; K Wachsmuth; O Olsvik
Journal:  J Clin Microbiol       Date:  1992-08       Impact factor: 5.948
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  9 in total

1.  Detection of Vibrio cholerae by real-time nucleic acid sequence-based amplification.

Authors:  Else M Fykse; Gunnar Skogan; William Davies; Jaran Strand Olsen; Janet M Blatny
Journal:  Appl Environ Microbiol       Date:  2007-01-12       Impact factor: 4.792

2.  Detection, isolation, and identification of Vibrio cholerae from the environment.

Authors:  Anwar Huq; Bradd J Haley; Elisa Taviani; Arlene Chen; Nur A Hasan; Rita R Colwell
Journal:  Curr Protoc Microbiol       Date:  2012-08

3.  Direct and Rapid Identification of Vibrio Cholerae Serogroup and Toxigenicity by a Novel Multiplex Real-Time Assay.

Authors:  Yong Yan; Li Zhan; Guoying Zhu; Junyan Zhang; Ping Li; Lixia Chen; Peiyan He; Jianyong Luo; Zhongwen Chen
Journal:  Pathogens       Date:  2022-07-30

4.  Simultaneous Screening of 24 Target Genes of Foodborne Pathogens in 35 Foodborne Outbreaks Using Multiplex Real-Time SYBR Green PCR Analysis.

Authors:  Hiroshi Fukushima; Jun Kawase; Yoshiki Etoh; Kumiko Sugama; Shunshuke Yashiro; Natsuko Iida; Keiji Yamaguchi
Journal:  Int J Microbiol       Date:  2010-09-28

5.  Quadruplex real-time PCR assay for detection and identification of Vibrio cholerae O1 and O139 strains and determination of their toxigenic potential.

Authors:  Jianwei Huang; Yumei Zhu; Huixin Wen; Jiafeng Zhang; Shijie Huang; Jianjun Niu; Qingge Li
Journal:  Appl Environ Microbiol       Date:  2009-09-18       Impact factor: 4.792

6.  Molecular beacons in diagnostics.

Authors:  Sanjay Tyagi; Fred Russell Kramer
Journal:  F1000 Med Rep       Date:  2012-05-02

7.  Molecular Characterization of Vibrio cholerae Isolated From Clinical Samples in Kurdistan Province, Iran.

Authors:  Rashid Ramazanzadeh; Samaneh Rouhi; Pegah Shakib; Babak Shahbazi; Farzam Bidarpour; Mohammad Karimi
Journal:  Jundishapur J Microbiol       Date:  2015-05-31       Impact factor: 0.747

8.  Development and Validation of a Novel Real-time Assay for the Detection and Quantification of Vibrio cholerae.

Authors:  Ridwan Bin Rashid; Jannatul Ferdous; Suhella Tulsiani; Peter Kjaer Mackie Jensen; Anowara Begum
Journal:  Front Public Health       Date:  2017-05-19

9.  Spatiotemporal Dynamics of Vibrio spp. within the Sydney Harbour Estuary.

Authors:  Nachshon Siboni; Varunan Balaraju; Richard Carney; Maurizio Labbate; Justin R Seymour
Journal:  Front Microbiol       Date:  2016-04-12       Impact factor: 5.640
  9 in total

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