Literature DB >> 12409365

Collaborative evaluation of optimal antifungal susceptibility testing conditions for dermatophytes.

Belkys Fernández-Torres1, Francisco J Cabañes, Alfonso J Carrillo-Muñoz, Alexandre Esteban, Isabel Inza, Lourdes Abarca, Josep Guarro.   

Abstract

A multicenter study was conducted to define the most suitable testing conditions for antifungal susceptibility of dermatophytes. Broth microdilution MICs of clotrimazole, itraconazole, and terbinafine were determined in three centers against 60 strains of dermatophytes. The effects of inoculum density (ca. 10(3) and 10(4) CFU/ml), incubation time (3, 7, and 14 days), endpoint criteria for MIC determination (complete [MIC-0] and prominent [MIC-2] growth inhibition), and incubation temperature (28 and 37 degrees C) on intra- and interlaboratory agreement were analyzed. The optimal testing conditions identified were an inoculum of 10(4) CFU/ml, a temperature of incubation of 28 degrees C, an incubation period of 7 days, and MIC-0.

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Year:  2002        PMID： 12409365      PMCID： PMC139645          DOI： 10.1128/JCM.40.11.3999-4003.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  32 in total

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Journal:  Antimicrob Agents Chemother       Date:  1995-05       Impact factor: 5.191

6.  Antimycotic susceptibility testing of dermatophytes in microcultures with a standardized fragmented mycelial inoculum.

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Authors:  S Perea; A W Fothergill; D A Sutton; M G Rinaldi
Journal:  J Clin Microbiol       Date:  2001-01       Impact factor: 5.948

Review 9.  Role of oral antifungal agents for the treatment of superficial fungal infections in immunocompromised patients.

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Journal:  Cutis       Date:  2001-07

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  24 in total

1.  Evaluation of disk diffusion method for determining eberconazole susceptibility of dermatophytes and influence of culture medium.

Authors:  Belkys Fernández-Torres; Isabel Inza; Josep Guarro
Journal:  Antimicrob Agents Chemother       Date:  2005-05       Impact factor: 5.191

2.  Establishing a method of inoculum preparation for susceptibility testing of Trichophyton rubrum and Trichophyton mentagrophytes.

Authors:  D A Santos; M E S Barros; J S Hamdan
Journal:  J Clin Microbiol       Date:  2006-01       Impact factor: 5.948

3.  Effect of culture medium on the disk diffusion method for determining antifungal susceptibilities of dermatophytes.

Authors:  Belkys Fernández-Torres; Alfonso Carrillo-Muñoz; Isabel Inza; Josep Guarro
Journal:  Antimicrob Agents Chemother       Date:  2006-06       Impact factor: 5.191

4.  Evaluation of broth microdilution antifungal susceptibility testing conditions for Trichophyton rubrum.

Authors:  D A Santos; J S Hamdan
Journal:  J Clin Microbiol       Date:  2005-04       Impact factor: 5.948

5.  Standardization of hyphal growth inhibition rate as a means of evaluating Microsporum spp. in vitro susceptibility to terbinafine, griseofulvin, and ciclopiroxolamine.

Authors:  Fernanda Simas Corrêa Biancalana; Luzia Lyra; Maria Luiza Moretti; Katsuhiko Kamei; Angélica Zaninelli Schreiber
Journal:  Mycopathologia       Date:  2011-05-25       Impact factor: 2.574

6.  Comparison of the in vitro activities of newer triazoles and established antifungal agents against Trichophyton rubrum.

Authors:  Shuwen Deng; Chao Zhang; Seyedmojtaba Seyedmousavi; Shuang Zhu; Xin Tan; Yiyang Wen; Xin Huang; Wenzhi Lei; Zhaojing Zhou; Wenjie Fang; Shuaishuai Shen; Danqi Deng; Weihua Pan; Wanqing Liao
Journal:  Antimicrob Agents Chemother       Date:  2015-04-20       Impact factor: 5.191

7.  Transcriptional profiles of the response to ketoconazole and amphotericin B in Trichophyton rubrum.

Authors:  Lu Yu; Wenliang Zhang; Lingling Wang; Jian Yang; Tao Liu; Junping Peng; Wenchuan Leng; Lihong Chen; Ruoyu Li; Qi Jin
Journal:  Antimicrob Agents Chemother       Date:  2006-10-23       Impact factor: 5.191

8.  In vitro activities of miltefosine and two novel antifungal biscationic salts against a panel of 77 dermatophytes.

Authors:  Zhongsheng Tong; Fred Widmer; Tania C Sorrell; Zofia Guse; Katrina A Jolliffe; Catriona Halliday; Ok Cha Lee; Fanrong Kong; Lesley C Wright; Sharon C A Chen
Journal:  Antimicrob Agents Chemother       Date:  2007-03-19       Impact factor: 5.191

9.  In vitro susceptibility testing of Microsporum gypseum isolated from healthy cattle and soil samples against itraconazole, terbinafine, fluconazole and topical veterinarian drugs.

Authors:  Alessandra Gonçalves Krakhecke; Eurípedes Afonso; Joseane C Ferreira; Regina Celia Candido
Journal:  Mycopathologia       Date:  2005-04       Impact factor: 2.574

10.  Molecular Characterization and In Vitro Antifungal Susceptibility of 316 Clinical Isolates of Dermatophytes in Iran.

Authors:  Saham Ansari; Mohammad T Hedayati; Kamiar Zomorodian; Keyvan Pakshir; Hamid Badali; Abdollah Rafiei; Mostafa Ravandeh; Seyedmojtaba Seyedmousavi
Journal:  Mycopathologia       Date:  2015-09-14       Impact factor: 2.574
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