AIM: The purpose of this study was to characterize the dentine surface of the pulpal floor and to determine the micro-tensile bond strengths of two dentine adhesive systems used in conjunction with a resin composite core material to pulpal floor dentine. METHODOLOGY:Thirty-six extracted human molars were cut horizontally with a slow-speed diamond saw to expose the pulp chamber and the pulp tissue was removed. Eight teeth were used to investigate characteristics of untreated and etched dentine surfaces of the pulpal floor using SEM. Twenty-eight teeth were randomly allocated to two groups, each of 14 teeth. In group 1, a 'one-bottle' system (Prime & Bond NT) was used as dentine bonding agent and in group 2, a self-etching-priming system (Clearfil SE Bond) was applied. Pulp chambers of both groups were then filled with resin composite core buildup (FluoroCore) and kept moist in tap water at 37 degrees C for 24 h. Samples were prepared for micro-tensile bond testing by sectioning each tooth vertically in the mid-pulpal floor region and shaped to an hour-glass form of 1 +/- 0.2 mm(2) area at the bonded interface. Micro-tensile bond strengths were determined using a universal testing machine at a crosshead speed of 1 mm min-1. Fractured surfaces were examined by SEM. RESULTS: The intact pulpal floor showed numerous calcospherites with variable tubule density and few accessory canals. Etching the pulpal floor with 34% phosphoric acid gel resulted in an irregular surface with patent dentinal tubules and the dome-shaped calcospherites mostly lost. With self-etching primer, the superficial dentine surface was less demineralized and little peritubular dentine matrix was removed. Mean bond strength for the self-etching-priming (Clearfil SE Bond) specimens was significantly greater than for 'one-bottle' (Prime & Bond NT) specimens (P< 0.05, Student's t-test). The fracture mode of Clearfil SE Bond specimens was mostly partial cohesive failure within dentine, whilst Prime & Bond NT specimens mostly showed partial cohesive failure in bonding resin/composite. CONCLUSIONS: Despite an irregular surface morphology and absence of a smear layer, bonding to pulpal floor dentine was weaker than previously reported for cut coronal dentine. The self-etching-priming system bonded more strongly than the 'one-bottle' system.
RCT Entities:
AIM: The purpose of this study was to characterize the dentine surface of the pulpal floor and to determine the micro-tensile bond strengths of two dentine adhesive systems used in conjunction with a resin composite core material to pulpal floor dentine. METHODOLOGY: Thirty-six extracted human molars were cut horizontally with a slow-speed diamond saw to expose the pulp chamber and the pulp tissue was removed. Eight teeth were used to investigate characteristics of untreated and etched dentine surfaces of the pulpal floor using SEM. Twenty-eight teeth were randomly allocated to two groups, each of 14 teeth. In group 1, a 'one-bottle' system (Prime & Bond NT) was used as dentine bonding agent and in group 2, a self-etching-priming system (Clearfil SE Bond) was applied. Pulp chambers of both groups were then filled with resin composite core buildup (FluoroCore) and kept moist in tap water at 37 degrees C for 24 h. Samples were prepared for micro-tensile bond testing by sectioning each tooth vertically in the mid-pulpal floor region and shaped to an hour-glass form of 1 +/- 0.2 mm(2) area at the bonded interface. Micro-tensile bond strengths were determined using a universal testing machine at a crosshead speed of 1 mm min-1. Fractured surfaces were examined by SEM. RESULTS: The intact pulpal floor showed numerous calcospherites with variable tubule density and few accessory canals. Etching the pulpal floor with 34% phosphoric acid gel resulted in an irregular surface with patent dentinal tubules and the dome-shaped calcospherites mostly lost. With self-etching primer, the superficial dentine surface was less demineralized and little peritubular dentine matrix was removed. Mean bond strength for the self-etching-priming (Clearfil SE Bond) specimens was significantly greater than for 'one-bottle' (Prime & Bond NT) specimens (P< 0.05, Student's t-test). The fracture mode of Clearfil SE Bond specimens was mostly partial cohesive failure within dentine, whilst Prime & Bond NT specimens mostly showed partial cohesive failure in bonding resin/composite. CONCLUSIONS: Despite an irregular surface morphology and absence of a smear layer, bonding to pulpal floor dentine was weaker than previously reported for cut coronal dentine. The self-etching-priming system bonded more strongly than the 'one-bottle' system.