Literature DB >> 12393155

Melting curve analysis of feline calicivirus isolates detected by real-time reverse transcription PCR.

Chris Helps1, Philippa Lait, Séverine Tasker, Dave Harbour.   

Abstract

A real-time reverse transcription polymerase chain reaction assay was developed to detect feline calicivirus infection in cats. Using SYBR green I melting curve analysis, isolates could be distinguished by their melting temperature. The assay is sensitive, linear over a wide range of template concentrations and allows accurate quantitation of viral load. Copyright 2002 Elsevier Science B.V.

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Year:  2002        PMID： 12393155     DOI： 10.1016/s0166-0934(02)00167-2

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  18 in total

1.  Feline coronavirus serotypes 1 and 2: seroprevalence and association with disease in Switzerland.

Authors:  Maya Kummrow; Marina L Meli; Michael Haessig; Enikoe Goenczi; Amy Poland; Niels C Pedersen; Regina Hofmann-Lehmann; Hans Lutz
Journal:  Clin Diagn Lab Immunol       Date:  2005-10

2.  Rapid, sensitive, and discriminating identification of Naegleria spp. by real-time PCR and melting-curve analysis.

Authors:  Bret S Robinson; Paul T Monis; Phillip J Dobson
Journal:  Appl Environ Microbiol       Date:  2006-09       Impact factor: 4.792

3.  Seroprevalences to viral pathogens in free-ranging and captive cheetahs (Acinonyx jubatus) on Namibian Farmland.

Authors:  Susanne Thalwitzer; Bettina Wachter; Nadia Robert; Gudrun Wibbelt; Thomas Müller; Johann Lonzer; Marina L Meli; Gert Bay; Heribert Hofer; Hans Lutz
Journal:  Clin Vaccine Immunol       Date:  2009-12-02

4.  Virucidal Efficacy of a Hydrogen Peroxide Nebulization Against Murine Norovirus and Feline Calicivirus, Two Surrogates of Human Norovirus.

Authors:  William Zonta; Axel Mauroy; Frederic Farnir; Etienne Thiry
Journal:  Food Environ Virol       Date:  2016-07-06       Impact factor: 2.778

5.  Exposure of cats to low doses of FeLV: seroconversion as the sole parameter of infection.

Authors:  Andrea Major; Valentino Cattori; Eva Boenzli; Barbara Riond; Peter Ossent; Marina Luisa Meli; Regina Hofmann-Lehmann; Hans Lutz
Journal:  Vet Res       Date:  2009-10-28       Impact factor: 3.683

6.  In vivo transmission studies of 'Candidatus Mycoplasma turicensis' in the domestic cat.

Authors:  Kristina Museux; Felicitas S Boretti; Barbara Willi; Barbara Riond; Katharina Hoelzle; Ludwig E Hoelzle; Max M Wittenbrink; Séverine Tasker; Nicole Wengi; Claudia E Reusch; Hans Lutz; Regina Hofmann-Lehmann
Journal:  Vet Res       Date:  2009-05-16       Impact factor: 3.683

7.  Feline leukemia virus and other pathogens as important threats to the survival of the critically endangered Iberian lynx (Lynx pardinus).

Authors:  Marina L Meli; Valentino Cattori; Fernando Martínez; Guillermo López; Astrid Vargas; Miguel A Simón; Irene Zorrilla; Alvaro Muñoz; Francisco Palomares; Jose V López-Bao; Josep Pastor; Ravi Tandon; Barbara Willi; Regina Hofmann-Lehmann; Hans Lutz
Journal:  PLoS One       Date:  2009-03-09       Impact factor: 3.240

8.  Use of DNA melting simulation software for in silico diagnostic assay design: targeting regions with complex melting curves and confirmation by real-time PCR using intercalating dyes.

Authors:  John P Rasmussen; Christopher P Saint; Paul T Monis
Journal:  BMC Bioinformatics       Date:  2007-03-29       Impact factor: 3.169

9.  A SYBR Green RT-PCR assay in single tube to detect human and bovine noroviruses and control for inhibition.

Authors:  Alexandra Scipioni; Axel Mauroy; Dominique Ziant; Claude Saegerman; Etienne Thiry
Journal:  Virol J       Date:  2008-08-14       Impact factor: 4.099

10.  Feline calicivirus and other respiratory pathogens in cats with Feline calicivirus-related symptoms and in clinically healthy cats in Switzerland.

Authors:  Alice Berger; Barbara Willi; Marina L Meli; Felicitas S Boretti; Sonja Hartnack; Anou Dreyfus; Hans Lutz; Regina Hofmann-Lehmann
Journal:  BMC Vet Res       Date:  2015-11-13       Impact factor: 2.741
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