Literature DB >> 12382669

Evidence for hepatocyte differentiation from embryonic stem cells in vitro.

Hitoshi Miyashita1, Atsushi Suzuki, Katashi Fukao, Hiromitsu Nakauchi, Hideki Taniguchi.   

Abstract

We confirmed hepatocyte differentiation from embryonic stem (ES) cells in vitro. RT-PCR analysis revealed that a broad range of hepatic gene expression was observed in ES cells differentiated through formation of embryoid bodies (EBs) and its attachment culture. Quantitative PCR analysis revealed that hepatic gene expression related to early and late-stage liver development were enhanced through in vitro differentiation of ES cells. The presence of albumin-producing cells in the peripheral region of attached EBs was confirmed by immunocytochemical analysis. Future experiments will reveal the molecules that induce hepatocyte differentiation from ES cells in vitro. This research will provide systems for the investigation of mechanisms in liver development and establish a method of ES cell-based therapy for liver diseases.

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Year:  2002        PMID： 12382669

Source DB:  PubMed          Journal:  Cell Transplant        ISSN： 0963-6897            Impact factor:   4.064


  11 in total

1.  Model systems and clinical applications of hepatic stem cells for liver regeneration.

Authors:  Min Zhang; Yan Zhong; Jun Chen
Journal:  Hepatol Int       Date:  2011-12-21       Impact factor: 6.047

2.  Recellularization of rat liver scaffolds by human liver stem cells.

Authors:  Victor Navarro-Tableros; Maria Beatriz Herrera Sanchez; Federico Figliolini; Renato Romagnoli; Ciro Tetta; Giovanni Camussi
Journal:  Tissue Eng Part A       Date:  2015-04-29       Impact factor: 3.845

3.  Embryonic stem cells develop into hepatocytes after intrasplenic transplantation in CCl4-treated mice.

Authors:  Kei Moriya; Masahide Yoshikawa; Ko Saito; Yukiteru Ouji; Mariko Nishiofuku; Noriko Hayashi; Shigeaki Ishizaka; Hiroshi Fukui
Journal:  World J Gastroenterol       Date:  2007-02-14       Impact factor: 5.742

4.  Promoted differentiation of cynomolgus monkey ES cells into hepatocyte-like cells by co-culture with mouse fetal liver-derived cells.

Authors:  Ko Saito; Masahide Yoshikawa; Yukiteru Ouji; Kei Moriya; Mariko Nishiofuku; Shigehiko Ueda; Noriko Hayashi; Shigeaki Ishizaka; Hiroshi Fukui
Journal:  World J Gastroenterol       Date:  2006-11-14       Impact factor: 5.742

5.  Embryoid body-mediated differentiation of mouse embryonic stem cells along a hepatocyte lineage: insights from gene expression profiles.

Authors:  Eric I Novik; Tim J Maguire; Ksenia Orlova; Rene S Schloss; Martin L Yarmush
Journal:  Tissue Eng       Date:  2006-06

6.  Augmentation of EB-directed hepatocyte-specific function via collagen sandwich and SNAP.

Authors:  Eric I Novik; Jeffery Barminko; Tim J Maguire; Nripen Sharma; Eric J Wallenstein; Rene S Schloss; Martin L Yarmush
Journal:  Biotechnol Prog       Date:  2008 Sep-Oct

Review 7.  The role of stem cells in physiology, pathophysiology, and therapy of the liver.

Authors:  Amar Deep Sharma; Tobias Cantz; Michael P Manns; Michael Ott
Journal:  Stem Cell Rev       Date:  2006       Impact factor: 6.692

Review 8.  Stem cells in liver regeneration and therapy.

Authors:  Tobias Cantz; Michael P Manns; Michael Ott
Journal:  Cell Tissue Res       Date:  2007-09-28       Impact factor: 5.249

9.  Functional comparison of human embryonic stem cells and induced pluripotent stem cells as sources of hepatocyte-like cells.

Authors:  Jaemin Jeong; Kyu Nam Kim; Min Sung Chung; Han Joon Kim
Journal:  Tissue Eng Regen Med       Date:  2016-12-17       Impact factor: 4.169

10.  Human liver stem cell-derived microvesicles accelerate hepatic regeneration in hepatectomized rats.

Authors:  M B Herrera; V Fonsato; S Gatti; M C Deregibus; A Sordi; D Cantarella; R Calogero; B Bussolati; C Tetta; G Camussi
Journal:  J Cell Mol Med       Date:  2009-07-24       Impact factor: 5.310
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