AIM: To investigate the effect of N-desulfated heparin on hepatic/renal ischemia and reperfusion injury in rats. METHODS: Using rat models of 60 minutes hepatic or renal ischemia followed by 1 h,3 h,6 h and 24 h reperfusion,animals were randomly divided into following groups,the sham operated controls,ischemic group receiving only normal saline,and treated group receiving N-desulfated heparin at a dose of 12 mg/kg at 5 minutes before reperfusion. P-selectin expression was detected in hepatic/renal tissues with immunohistochemistry method. RESULTS: P-selectin expression, serum ALT, AST, BUN and Cr levels were significantly increased during 60 minute ischemia and 1 h, 3 h, 6 h and 24 h reperfusion,while the increment was significantly inhibited,and hepatic/renal pathology observed by light microscopy was remarkably improved by treatment with the N-desulfated heparin. Furthermore,the heparin was found no effects on PT and KPTT. CONCLUSION: P-selectin might mediate neutrophil infiltration and contribute to hepatic/renal ischemia and reperfusion. The N-desulfated heparin might prevent hepatic/renal damage induced by ischemia and reperfusion injury without significant anticoagulant activity.
AIM: To investigate the effect of N-desulfated heparin on hepatic/renal ischemia and reperfusion injury in rats. METHODS: Using rat models of 60 minutes hepatic or renal ischemia followed by 1 h,3 h,6 h and 24 h reperfusion,animals were randomly divided into following groups,the sham operated controls,ischemic group receiving only normal saline,and treated group receiving N-desulfated heparin at a dose of 12 mg/kg at 5 minutes before reperfusion. P-selectin expression was detected in hepatic/renal tissues with immunohistochemistry method. RESULTS:P-selectin expression, serum ALT, AST, BUN and Cr levels were significantly increased during 60 minute ischemia and 1 h, 3 h, 6 h and 24 h reperfusion,while the increment was significantly inhibited,and hepatic/renal pathology observed by light microscopy was remarkably improved by treatment with the N-desulfated heparin. Furthermore,the heparin was found no effects on PT and KPTT. CONCLUSION:P-selectin might mediate neutrophil infiltration and contribute to hepatic/renal ischemia and reperfusion. The N-desulfated heparin might prevent hepatic/renal damage induced by ischemia and reperfusion injury without significant anticoagulant activity.
Authors: I Singh; G B Zibari; M F Brown; D N Granger; M Eppihimer; H Zizzi; L Cruz; K Meyer; E Gonzales; J C McDonald Journal: Clin Transplant Date: 1999-02 Impact factor: 2.863
Authors: H C Zizzi; G B Zibari; D N Granger; I Singh; L D Cruz; F Abreo; J C McDonald; M F Brown Journal: J Pediatr Surg Date: 1997-07 Impact factor: 2.545
Authors: X Xie; A S Rivier; A Zakrzewicz; M Bernimoulin; X L Zeng; H P Wessel; M Schapira; O Spertini Journal: J Biol Chem Date: 2000-11-03 Impact factor: 5.157
Authors: Enio Rodrigues Vasques; Jose Eduardo Monteiro Cunha; Ana Maria Mendonca Coelho; Sandra N Sampietre; Rosely Antunes Patzina; Emilio Elias Abdo; Helena B Nader; Ivarne L S Tersariol; Marcelo Andrade Lima; Carlos M G Godoy; Tiago Rodrigues; Eleazar Chaib; Luiz A C D'Albuquerque Journal: PLoS One Date: 2016-02-22 Impact factor: 3.240