T Zhou1, X Li, P Wu, D Zhang, M Zhang, N Chen, D Dong. 1. Department of Nephrology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.
Abstract
OBJECTIVE: To explore the effect of anti-P-selectin monoclonal antibody (mAb) on renal ischemia/reperfusion (I/R) injury in rats. METHODS: Renal function, renal histopathological changes, plasma P-selectin levels and renal P-selectin protein and mRNA expression were studied in a renal I/R injury rat models. Biochemical measurement, ELISA, Immunohistochemistry and Nested RT-PCR were used. RESULTS: Renal function insufficiency and renal histopathological damage were much less in the anti-P-selectin mAb-treated group than the saline-treated group. Plasma P-selectin levels were lower and renal P-selectin protein and mRNA expression were down-regulated in the former group. CONCLUSION: Anti-P-selectin mAb might be an efficient approach for the treatment of renal I/R injury.
OBJECTIVE: To explore the effect of anti-P-selectin monoclonal antibody (mAb) on renal ischemia/reperfusion (I/R) injury in rats. METHODS: Renal function, renal histopathological changes, plasma P-selectin levels and renal P-selectin protein and mRNA expression were studied in a renal I/R injury rat models. Biochemical measurement, ELISA, Immunohistochemistry and Nested RT-PCR were used. RESULTS:Renal function insufficiency and renal histopathological damage were much less in the anti-P-selectin mAb-treated group than the saline-treated group. Plasma P-selectin levels were lower and renal P-selectin protein and mRNA expression were down-regulated in the former group. CONCLUSION: Anti-P-selectin mAb might be an efficient approach for the treatment of renal I/R injury.