Literature DB >> 12351880

Agarose as crystallisation media for proteins II: trapping of gel fibres into the crystals.

José Antonio Gavira1, Juan Manuel García-Ruiz.   

Abstract

The crystallisation pressure exerted by protein crystals growing in agarose gel does not disrupt the gel network. However, protein crystals trap agarose fibres when they grow in agarose gels. The fibres of agarose are distributed randomly in the crystals explaining why they do not appreciably affect the diffraction quality of the crystal

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Year:  2002        PMID： 12351880     DOI： 10.1107/s0907444902014609

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  7 in total

Review 1.  Protein crystallization in the structural genomics era.

Authors:  Alexander McPherson
Journal:  J Struct Funct Genomics       Date:  2004

2.  Anaerobic crystallization and initial X-ray diffraction data of biphenyl 2,3-dioxygenase from Burkholderia xenovorans LB400: addition of agarose improved the quality of the crystals.

Authors:  Pravindra Kumar; Leticia Gómez-Gil; Mahmood Mohammadi; Michel Sylvestre; Lindsay D Eltis; Jeffrey T Bolin
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-12-21

3.  Crystallization of the large membrane protein complex photosystem I in a microfluidic channel.

Authors:  Bahige G Abdallah; Christopher Kupitz; Petra Fromme; Alexandra Ros
Journal:  ACS Nano       Date:  2013-11-14       Impact factor: 15.881

4.  Use of Cross-Linked Poly(ethylene glycol)-Based Hydrogels for Protein Crystallization.

Authors:  Jose A Gavira; Andry Cera-Manjarres; Katia Ortiz; Janet Mendez; Jose A Jimenez-Torres; Luis D Patiño-Lopez; Madeline Torres-Lugo
Journal:  Cryst Growth Des       Date:  2014-05-14       Impact factor: 4.076

5.  Three-dimensional, non-invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography.

Authors:  Norihiko Nishizawa; Shutaro Ishida; Mika Hirose; Shigeru Sugiyama; Tsuyoshi Inoue; Yusuke Mori; Kazuyoshi Itoh; Hiroyoshi Matsumura
Journal:  Biomed Opt Express       Date:  2012-03-15       Impact factor: 3.732

6.  A technique for high-throughput protein crystallization in ionically cross-linked polysaccharide gel beads for X-ray diffraction experiments.

Authors:  Michihiro Sugahara
Journal:  PLoS One       Date:  2014-04-16       Impact factor: 3.240

Review 7.  Sample Delivery Media for Serial Crystallography.

Authors:  Ki Hyun Nam
Journal:  Int J Mol Sci       Date:  2019-03-04       Impact factor: 5.923
  7 in total

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