Literature DB >> 12323353

Two-dimensional gel electrophoresis; better than a poke in the ICAT?

Wayne F Patton1, Birte Schulenberg, Thomas H Steinberg.   

Abstract

To date, the most widely used technology for conducting proteomic studies has been two-dimensional gel electrophoresis (2DGE), but this approach does have drawbacks. Isotope-coded affinity tagging (ICAT) is starting to challenge 2DGE as a new proteomic tool for the analysis of proteins in complex biological specimens. An appraisal of these two methodologies reveals that neither ICAT nor 2DGE provide comprehensive coverage on a proteome-wide scale.

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Year:  2002        PMID： 12323353     DOI： 10.1016/s0958-1669(02)00333-6

Source DB:  PubMed          Journal:  Curr Opin Biotechnol        ISSN： 0958-1669            Impact factor:   9.740


  15 in total

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4.  Proteomic analysis of peritrophic membrane (PM) from the midgut of fifth-instar larvae, Bombyx mori.

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5.  Utility of cleavable isotope-coded affinity-tagged reagents for quantification of low-copy proteins induced by methylprednisolone using liquid chromatography/tandem mass spectrometry.

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6.  Activity-Based Protein Profiling (ABPP) and Click Chemistry (CC)-ABPP by MudPIT Mass Spectrometry.

Authors:  Anna E Speers; Benjamin F Cravatt
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8.  MALDI-TOF and ESI-MS analysis of oligosaccharides labeled with a new multifunctional oligosaccharide tag.

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9.  Measurement of protein sulfhydryls in response to cellular oxidative stress using gel electrophoresis and multiplexed fluorescent imaging analysis.

Authors:  Page C Spiess; Dexter Morin; William T Jewell; Alan R Buckpitt
Journal:  Chem Res Toxicol       Date:  2008-04-17       Impact factor: 3.739

10.  Assessment of some tools for the characterization of the human osteoarthritic cartilage proteome.

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