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Literature DB >> 12323135

Hydrogen bonds in human ubiquitin reflected in temperature coefficients of amide protons.

Tomasz Cierpicki¹, Igor Zhukov, R Andrew Byrd, Jacek Otlewski.

Abstract

Analysis of amide proton temperature coefficients (deltasigma(HN)/DeltaT) in human ubiquitin shows their usefulness in indicating hydrogen bonds. The availability of a very accurate solution structure of ubiquitin enables the precise determination of hydrogen bonds and increases the reliability of the analysis of chemical shift temperature gradients. Values of deltasigma(HN)/DeltaT more positive than -4.6 ppb/K are very good indicators of hydrogen bonds. Additionally, a weak temperature dependence of non-hydrogen-bonded amides was observed for amide protons that are significantly shifted upfield. We observed that temperature gradients of amide protons involved in short hydrogen bonds are related to donor-acceptor distances.

Entities: Chemical Species

Mesh：

Substances：
Amides
Ubiquitins

Year: 2002 PMID： 12323135 DOI： 10.1006/jmre.2002.2597

Source DB: PubMed Journal: J Magn Reson ISSN： 1090-7807 Impact factor: 2.229

Keyword Cloud
Cited

21 in total

1. Amide temperature coefficients in the protein G B1 domain.

Authors: Jennifer H Tomlinson; Mike P Williamson
Journal: J Biomol NMR Date: 2011-11-11 Impact factor: 2.835

2. Importance of alpha-helix N-capping motif in stabilization of betabetaalpha fold.

Authors: Katarzyna Koscielska-Kasprzak; Tomasz Cierpicki; Jacek Otlewski
Journal: Protein Sci Date: 2003-06 Impact factor: 6.725

3. A Second Backbone: The Contribution of a Buried Asparagine Ladder to the Global and Local Stability of a Leucine-Rich Repeat Protein.

Authors: Sean A Klein; Ananya Majumdar; Doug Barrick
Journal: Biochemistry Date: 2019-08-06 Impact factor: 3.162

4. Using ¹ H^N amide temperature coefficients to define intrinsically disordered regions: An alternative NMR method.

Authors: Hiroki Okazaki; Naoki Matsuo; Takeshi Tenno; Natsuko Goda; Yoshiki Shigemitsu; Motonori Ota; Hidekazu Hiroaki
Journal: Protein Sci Date: 2018-10-03 Impact factor: 6.725

5. The protein amide ¹H(N) chemical shift temperature coefficient reflects thermal expansion of the N-H···O=C hydrogen bond.

Authors: Jingbo Hong; Qingqing Jing; Lishan Yao
Journal: J Biomol NMR Date: 2012-12-01 Impact factor: 2.835

Hydrogen bonds in human ubiquitin reflected in temperature coefficients of amide protons.

1. Amide temperature coefficients in the protein G B1 domain.

2. Importance of alpha-helix N-capping motif in stabilization of betabetaalpha fold.

3. A Second Backbone: The Contribution of a Buried Asparagine Ladder to the Global and Local Stability of a Leucine-Rich Repeat Protein.

4. Using ¹ H^N amide temperature coefficients to define intrinsically disordered regions: An alternative NMR method.

5. The protein amide ¹H(N) chemical shift temperature coefficient reflects thermal expansion of the N-H···O=C hydrogen bond.

6. Stability of Protein-Specific Hydration Shell on Crowding.

7. Mechanism of Protein Denaturation: Partial Unfolding of the P22 Coat Protein I-Domain by Urea Binding.

8. Measuring 1HN temperature coefficients in invisible protein states by relaxation dispersion NMR spectroscopy.

9. The novel fold of scytovirin reveals a new twist for antiviral entry inhibitors.

10. Dynamic Water-Mediated Hydrogen Bonding in a Collagen Model Peptide.