Literature DB >> 12234853

Mutations in the aph(2")-Ic gene are responsible for increased levels of aminoglycoside resistance.

Hae Kyung Lee1, Sergei B Vakulenko, Don B Clewell, Stephen A Lerner, Joseph W Chow.   

Abstract

Random PCR mutagenesis of the enterococcal aph(2")-Ic gene followed by selection for mutant enzymes that confer enhanced levels of aminoglycoside resistance resulted in mutants of APH(2")-Ic with His-258-Leu and Phe-108-Leu substitutions, all of which conferred rises in the MICs of several aminoglycosides. The mutated residues are located outside conserved regions of aminoglycoside phosphotransferases.

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Year:  2002        PMID： 12234853      PMCID： PMC128768          DOI： 10.1128/AAC.46.10.3253-3256.2002

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  17 in total

1.  Active site labeling of the gentamicin resistance enzyme AAC(6')-APH(2") by the lipid kinase inhibitor wortmannin.

Authors:  D D Boehr; W S Lane; G D Wright
Journal:  Chem Biol       Date:  2001-08

2.  Detection of the high-level aminoglycoside resistance gene aph(2")-Ib in Enterococcus faecium.

Authors:  S J Kao; I You; D B Clewell; S M Donabedian; M J Zervos; J Petrin; K J Shaw; J W Chow
Journal:  Antimicrob Agents Chemother       Date:  2000-10       Impact factor: 5.191

3.  Site-specific mutations of conserved C-terminal residues in aminoglycoside 3'-phosphotransferase II: phenotypic and structural analysis of mutant enzymes.

Authors:  S Kocabiyik; M H Perlin
Journal:  Biochem Biophys Res Commun       Date:  1992-06-30       Impact factor: 3.575

4.  Mutations in the aphA-2 gene of transposon Tn5 mapping within the regions highly conserved in aminoglycoside-phosphotransferases strongly reduce aminoglycoside resistance.

Authors:  J Blázquez; J Davies; F Moreno
Journal:  Mol Microbiol       Date:  1991-06       Impact factor: 3.501

5.  Nucleotide sequence analysis of the gene specifying the bifunctional 6'-aminoglycoside acetyltransferase 2"-aminoglycoside phosphotransferase enzyme in Streptococcus faecalis and identification and cloning of gene regions specifying the two activities.

Authors:  J J Ferretti; K S Gilmore; P Courvalin
Journal:  J Bacteriol       Date:  1986-08       Impact factor: 3.490

6.  A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure.

Authors:  R L Yenofsky; M Fine; J W Pellow
Journal:  Proc Natl Acad Sci U S A       Date:  1990-05       Impact factor: 11.205

7.  The COOH terminus of aminoglycoside phosphotransferase (3')-IIIa is critical for antibiotic recognition and resistance.

Authors:  P R Thompson; J Schwartzenhauer; D W Hughes; A M Berghuis; G D Wright
Journal:  J Biol Chem       Date:  1999-10-22       Impact factor: 5.157

8.  Nucleotide sequence of Acinetobacter baumannii aphA-6 gene: evolutionary and functional implications of sequence homologies with nucleotide-binding proteins, kinases and other aminoglycoside-modifying enzymes.

Authors:  P Martin; E Jullien; P Courvalin
Journal:  Mol Microbiol       Date:  1988-09       Impact factor: 3.501

9.  Altered substrate specificity by substitutions at Tyr218 in bacterial aminoglycoside 3'-phosphotransferase-II.

Authors:  S Kocabiyik; M H Perlin
Journal:  FEMS Microbiol Lett       Date:  1992-06-01       Impact factor: 2.742

Review 10.  Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes.

Authors:  K J Shaw; P N Rather; R S Hare; G H Miller
Journal:  Microbiol Rev       Date:  1993-03
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  2 in total

1.  Aminoglycoside 2''-phosphotransferase IIIa (APH(2'')-IIIa) prefers GTP over ATP: structural templates for nucleotide recognition in the bacterial aminoglycoside-2'' kinases.

Authors:  Clyde A Smith; Marta Toth; Hilary Frase; Laura J Byrnes; Sergei B Vakulenko
Journal:  J Biol Chem       Date:  2012-02-24       Impact factor: 5.157

2.  Plasticity of Aminoglycoside Binding to Antibiotic Kinase APH(2″)-Ia.

Authors:  Shane J Caldwell; Albert M Berghuis
Journal:  Antimicrob Agents Chemother       Date:  2018-06-26       Impact factor: 5.191
  2 in total

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