OBJECTIVE: Obesity is associated with increased morbidity and mortality from atherosclerotic disease. Lipid abnormalities contribute to the increased relative risk in obese subjects. Cholesteryl ester transfer protein (CETP) mass is increased in these patients and might mediate the atherogenic lipoprotein pattern observed in obesity. METHODS AND RESULTS: Twenty-one morbidly obese, middle-aged, female subjects participated in this prospective study. Subjects were examined before and 1 year after surgical treatment. Fat mass was determined by body impedance analysis; CETP mass, by ELISA; CETP activity, by exogenous substrate assay; and LDL particle diameter, by gradient gel electrophoresis. Mean weight loss after 1 year was 28.7 kg; mean fat mass loss was 22.6 kg. Mean CETP mass decreased from 1.81 to 1.32 microg/mL (P=0.008); mean CETP activity decreased from 244 to 184 nmol x mL(-1) x h(-1) (P=0.004); and in parallel, the mean diameter of LDL particles increased (256.8 to 258.4 A, P=0.04). CONCLUSIONS: We conclude that weight loss is associated with a pronounced decrease in CETP mass and activity and a consistent increase in LDL particle diameter. After 1 year of this prospective study in morbidly obese subjects undergoing weight loss by surgical treatment, it has been determined that some features of the atherogenic lipoprotein profile can be reversed.
OBJECTIVE:Obesity is associated with increased morbidity and mortality from atherosclerotic disease. Lipid abnormalities contribute to the increased relative risk in obese subjects. Cholesteryl ester transfer protein (CETP) mass is increased in these patients and might mediate the atherogenic lipoprotein pattern observed in obesity. METHODS AND RESULTS: Twenty-one morbidly obese, middle-aged, female subjects participated in this prospective study. Subjects were examined before and 1 year after surgical treatment. Fat mass was determined by body impedance analysis; CETP mass, by ELISA; CETP activity, by exogenous substrate assay; and LDL particle diameter, by gradient gel electrophoresis. Mean weight loss after 1 year was 28.7 kg; mean fat mass loss was 22.6 kg. Mean CETP mass decreased from 1.81 to 1.32 microg/mL (P=0.008); mean CETP activity decreased from 244 to 184 nmol x mL(-1) x h(-1) (P=0.004); and in parallel, the mean diameter of LDL particles increased (256.8 to 258.4 A, P=0.04). CONCLUSIONS: We conclude that weight loss is associated with a pronounced decrease in CETP mass and activity and a consistent increase in LDL particle diameter. After 1 year of this prospective study in morbidly obese subjects undergoing weight loss by surgical treatment, it has been determined that some features of the atherogenic lipoprotein profile can be reversed.
Authors: Mario Kratz; David S Weigle; Patricia A Breen; Kaatje E Meeuws; Verna R Burden; Holly S Callahan; Colleen C Matthys; Jonathan Q Purnell Journal: J Investig Med Date: 2010-06 Impact factor: 2.895
Authors: Markus W Laimer; Julia Engl; Alexander Tschoner; Susanne Kaser; Andreas Ritsch; Tobias Tatarczyk; Markus Rauchenzauner; Helmut Weiss; Franz Aigner; Josef R Patsch; Christoph F Ebenbichler Journal: Lipids Date: 2009-09-30 Impact factor: 1.880
Authors: Ezequiel Lozano Chiappe; Maximiliano Martin; Andrea Iglesias Molli; Andrea Millan; Walter Tetzlaff; Eliana Botta; Florencia Ferraro; Maria S Sáez; Maria V Lorenzon Gonzalez; Laura Boero; Patricia Sorroche; Axel Beskow; Mercedes Gutierrez; Gloria Cerrone; Susana Gutt; Gustavo Frechtel; Fernando Brites Journal: Obes Surg Date: 2020-10-30 Impact factor: 4.129