| Literature DB >> 12227746 |
Vince Pozsgay1, Nancy E Vieira, Alfred Yergey.
Abstract
Diels-Alder-type cycloaddition of an electronically matched pair of saccharide-linked conjugated dienes and a dienophile-equipped protein gives neoglycoproteins at ambient temperature in pure water with a reaction half-life of approximately 2 h. Uncoupled saccharides can be recovered by diafiltration with complete conservation of the diene moiety, thus allowing their repeated use. The procedure described is the first for creating a carbon-carbon covalent bond in the bioconjugation step between a saccharide and a protein.Entities:
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Year: 2002 PMID： 12227746 DOI： 10.1021/ol026179v
Source DB: PubMed Journal: Org Lett ISSN： 1523-7052 Impact factor: 6.005