| Literature DB >> 27876681 |
Min Shen1, Chandra K Dixit1, James Rusling1,2,3,4.
Abstract
Immobilized antibody systems are the key to develop efficient diagnostics and separations tools. In the last decade, developments in the field of biomolecular engineering and crosslinker chemistry have greatly influenced the development of this field. With all these new approaches at our disposal, several new immobilization methods have been created to address the main challenges associated with immobilized antibodies. Few of these challenges that we have discussed in this review are mainly associated to the site-specific immobilization, appropriate orientation, and activity retention. We have discussed the effect of antibody immobilization approaches on the parameters on the performance of an immunoassay.Entities:
Keywords: Affinity tags; Antibody; Antibody fragments; Antibody-binding protein; Click chemistry; Cycloaddition; Diagnostics; Immobilization; Metal binding protein; Nucleotide binding protein; Orientation; Physical adsorption; Protein A; Separation; Site-selective; Site-specific; Staudinger ligation; Therapeutics
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Year: 2016 PMID： 27876681 PMCID： PMC5374010 DOI： 10.1016/j.ymeth.2016.11.010
Source DB: PubMed Journal: Methods ISSN： 1046-2023 Impact factor: 3.608