Literature DB >> 12217654

Measuring diffusion in cell membranes by fluorescence correlation spectroscopy.

Parijat Sengupta1, J Balaji, Sudipta Maiti.   

Abstract

Fluorescence Correlation Spectroscopy (FCS) can measure diffusion on the cell surface with unparalleled sensitivity. In appropriate situations, this can be the most sensitive and accurate method for measuring receptor interaction and oligomerization. Here we attempt to describe FCS in sufficient detail so that the reader is able to judge when there is a compelling reason to choose this technique, understand the basic theory behind it, construct a FCS spectrometer in the laboratory, and analyze the data to obtain a meaningful estimate of the physical parameters.

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Year:  2002        PMID： 12217654     DOI： 10.1016/s1046-2023(02)00096-8

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  21 in total

1.  Measurement of the attachment and assembly of small amyloid-β oligomers on live cell membranes at physiological concentrations using single-molecule tools.

Authors:  Suman Nag; Jiji Chen; J Irudayaraj; S Maiti
Journal:  Biophys J       Date:  2010-09-22       Impact factor: 4.033

2.  Steric Crowding of the Turn Region Alters the Tertiary Fold of Amyloid-β18-35 and Makes It Soluble.

Authors:  Muralidharan Chandrakesan; Debanjan Bhowmik; Bidyut Sarkar; Rajiv Abhyankar; Harwinder Singh; Mamata Kallianpur; Sucheta P Dandekar; Perunthiruthy K Madhu; Sudipta Maiti; Venus Singh Mithu
Journal:  J Biol Chem       Date:  2015-10-20       Impact factor: 5.157

3.  Detecting amyloid-beta aggregation with fiber-based fluorescence correlation spectroscopy.

Authors:  Kanchan Garai; Ruchi Sureka; S Maiti
Journal:  Biophys J       Date:  2007-01-19       Impact factor: 4.033

4.  Development of multifunctional heterocyclic Schiff base as a potential metal chelator: a comprehensive spectroscopic approach towards drug discovery.

Authors:  Manojkumar Jadhao; Chayan Das; Anoop Rawat; Himank Kumar; Ritika Joshi; Sudipta Maiti; Sujit Kumar Ghosh
Journal:  J Biol Inorg Chem       Date:  2016-11-07       Impact factor: 3.358

5.  Curcumin Dictates Divergent Fates for the Central Salt Bridges in Amyloid-β40 and Amyloid-β42.

Authors:  Bappaditya Chandra; Venus Singh Mithu; Debanjan Bhowmik; Anand Kant Das; Bankanidhi Sahoo; Sudipta Maiti; Perunthiruthy K Madhu
Journal:  Biophys J       Date:  2017-04-25       Impact factor: 4.033

6.  Viscosity-dependent diffusion of fluorescent particles using fluorescence correlation spectroscopy.

Authors:  Chanbae Jung; Jaeran Lee; Manil Kang; Sok Won Kim
Journal:  J Fluoresc       Date:  2014-09-23       Impact factor: 2.217

7.  Nature of the amyloid-beta monomer and the monomer-oligomer equilibrium.

Authors:  Suman Nag; Bidyut Sarkar; Arkarup Bandyopadhyay; Bankanidhi Sahoo; Varun K A Sreenivasan; Mamata Kombrabail; Chandrakesan Muralidharan; Sudipta Maiti
Journal:  J Biol Chem       Date:  2011-02-24       Impact factor: 5.157

8.  Membrane fluidity and lipid order in ternary giant unilamellar vesicles using a new bodipy-cholesterol derivative.

Authors:  Florly S Ariola; Zaiguo Li; Christine Cornejo; Robert Bittman; Ahmed A Heikal
Journal:  Biophys J       Date:  2009-04-08       Impact factor: 4.033

9.  The Retinitis Pigmentosa-Linked Mutations in Transmembrane Helix 5 of Rhodopsin Disrupt Cellular Trafficking Regardless of Oligomerization State.

Authors:  D Paul Mallory; Elizabeth Gutierrez; Margaret Pinkevitch; Christie Klinginsmith; William D Comar; Francis J Roushar; Jonathan P Schlebach; Adam W Smith; Beata Jastrzebska
Journal:  Biochemistry       Date:  2018-08-21       Impact factor: 3.162

10.  Single-molecule photobleaching: Instrumentation and applications.

Authors:  Simli Dey; Sudipta Maiti
Journal:  J Biosci       Date:  2018-07       Impact factor: 1.826
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