| Literature DB >> 12216117 |
Sang-Gun Ahn1, Ho-Shik Kim, Seong-Whan Jeong, Bo-Eun Kim, Hyangshuk Rhim, Jae-Yong Shim, Jin-Woo Kim, Jeong-Hwa Lee, In-Kyung Kim.
Abstract
We reported earlier that expression of Sox-4 was found to be elevated during prostaglandin (PG) A(2) and delta(12)-PGJ(2) induced apoptosis in human hepatocarcinoma Hep3B cells. In this study, the role of Sox-4 was examined using human Hep3B and HepG2 cell lines. Sox-4 induction by several apoptotic inducer such as A23187 (Ca(2+) ionophore) and etoposide (topoisomerase II inhibitor) and Sox-4 transfection into the cells were able to induce apoptosis as observed by the cellular DNA fragmentation. Antisense oligonucleotide of Sox-4 inhibited the induction of Sox-4 expression and blocked the formation of DNA fragmentation by PGA(2) and delta(12)-PGJ(2) in Hep3B and HepG2 cells. Sox-4-induced apoptosis was accompanied with caspase-1 activation indicating that caspase cascade was involved in this apoptotic pathway. These results indicate that Sox-4 is involved in Hep3B and HepG2 cells apoptosis as an important apoptotic mediator.Entities:
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Year: 2002 PMID： 12216117 DOI： 10.1038/emm.2002.34
Source DB: PubMed Journal: Exp Mol Med ISSN： 1226-3613 Impact factor: 8.718