Literature DB >> 12183629

The calicheamicin gene cluster and its iterative type I enediyne PKS.

Joachim Ahlert1, Erica Shepard, Natalia Lomovskaya, Emmanuel Zazopoulos, Alfredo Staffa, Brian O Bachmann, Kexue Huang, Leonid Fonstein, Anne Czisny, Ross E Whitwam, Chris M Farnet, Jon S Thorson.   

Abstract

The enediynes exemplify nature's ingenuity. We have cloned and characterized the biosynthetic locus coding for perhaps the most notorious member of the nonchromoprotein enediyne family, calicheamicin. This gene cluster contains an unusual polyketide synthase (PKS) that is demonstrated to be essential for enediyne biosynthesis. Comparison of the calicheamicin locus with the locus encoding the chromoprotein enediyne C-1027 reveals that the enediyne PKS is highly conserved among these distinct enediyne families. Contrary to previous hypotheses, this suggests that the chromoprotein and nonchromoprotein enediynes are generated by similar biosynthetic pathways.

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Year:  2002        PMID： 12183629     DOI： 10.1126/science.1072105

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  102 in total

1.  Polyketide synthase chemistry does not direct biosynthetic divergence between 9- and 10-membered enediynes.

Authors:  Geoff P Horsman; Yihua Chen; Jon S Thorson; Ben Shen
Journal:  Proc Natl Acad Sci U S A       Date:  2010-06-07       Impact factor: 11.205

2.  Rapid PCR amplification of minimal enediyne polyketide synthase cassettes leads to a predictive familial classification model.

Authors:  Wen Liu; Joachim Ahlert; Qunjie Gao; Evelyn Wendt-Pienkowski; Ben Shen; Jon S Thorson
Journal:  Proc Natl Acad Sci U S A       Date:  2003-10-03       Impact factor: 11.205

3.  Polyketide and non-ribosomal peptide synthases: falling together by coming apart.

Authors:  C Richard Hutchinson
Journal:  Proc Natl Acad Sci U S A       Date:  2003-03-11       Impact factor: 11.205

4.  Cloning, sequencing, and functional analysis of an iterative type I polyketide synthase gene cluster for biosynthesis of the antitumor chlorinated polyenone neocarzilin in "Streptomyces carzinostaticus".

Authors:  Miyuki Otsuka; Koji Ichinose; Isao Fujii; Yutaka Ebizuka
Journal:  Antimicrob Agents Chemother       Date:  2004-09       Impact factor: 5.191

5.  Complete set of glycosyltransferase structures in the calicheamicin biosynthetic pathway reveals the origin of regiospecificity.

Authors:  Aram Chang; Shanteri Singh; Kate E Helmich; Randal D Goff; Craig A Bingman; Jon S Thorson; George N Phillips
Journal:  Proc Natl Acad Sci U S A       Date:  2011-10-10       Impact factor: 11.205

Review 6.  The structural biology of enzymes involved in natural product glycosylation.

Authors:  Shanteri Singh; George N Phillips; Jon S Thorson
Journal:  Nat Prod Rep       Date:  2012-06-12       Impact factor: 13.423

Review 7.  Avoidance of suicide in antibiotic-producing microbes.

Authors:  Eric Cundliffe; Arnold L Demain
Journal:  J Ind Microbiol Biotechnol       Date:  2010-05-06       Impact factor: 3.346

8.  A biosynthetic pathway for BE-7585A, a 2-thiosugar-containing angucycline-type natural product.

Authors:  Eita Sasaki; Yasushi Ogasawara; Hung-Wen Liu
Journal:  J Am Chem Soc       Date:  2010-06-02       Impact factor: 15.419

9.  Robust in vitro activity of RebF and RebH, a two-component reductase/halogenase, generating 7-chlorotryptophan during rebeccamycin biosynthesis.

Authors:  Ellen Yeh; Sylvie Garneau; Christopher T Walsh
Journal:  Proc Natl Acad Sci U S A       Date:  2005-03-02       Impact factor: 11.205

10.  Biochemical determination of enzyme-bound metabolites: preferential accumulation of a programmed octaketide on the enediyne polyketide synthase CalE8.

Authors:  Katherine Belecki; Craig A Townsend
Journal:  J Am Chem Soc       Date:  2013-09-17       Impact factor: 15.419
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