OBJECTIVE: To estimate the specificity of an absorbed enzyme-linked immunosorbent assay kit for Johne's disease (JD) when used in mature cattle populations resident in northern Australia. DESIGN: Blood samples were collected from beef cattle in northern Queensland, the Northern Territory and northern Western Australia, and from dairy cattle in northern Queensland. The specificity of a serological test for JD was estimated by testing the blood samples with an absorbed ELISA kit. Further samples were collected from cattle with positive ELISA results to determine the presence or absence of infection with Mycobacterium avium subsp paratuberculosis. PROCEDURE: During 1995 and 1996, blood, tissue and gut contents were collected from beef cattle at abattoirs in Queensland and the Northern Territory; and blood and faecal samples were collected from dairy cattle in herds assessed to be most at risk for JD in northern Queensland. The blood samples were tested using an absorbed ELISA kit. Tissues and gut contents from beef cattle that had positive ELISA results were cultured for M. avium subsp paratuberculosis, and tissues were examined histologically. Faecal samples from dairy cattle with positive ELISA results were cultured for M. avium subsp paratuberculosis. RESULTS: Estimates of specificity for this absorbed ELISA in mature northern Australian cattle were 98.0% (97.0 to 98.8%, 95% CI) in beef cattle, and 98.3% (96.7 to 99.3%, 95% CI) in dairy cattle. CONCLUSION: Estimates of specificity in this study were lower for beef cattle from the Northern Territory and northern Western Australia and for dairy cattle from northern Queensland than those quoted from studies on cattle in southern Western Australia. This should be considered when serological testing using the JD ELISA is carried out on northern Australian cattle.
OBJECTIVE: To estimate the specificity of an absorbed enzyme-linked immunosorbent assay kit for Johne's disease (JD) when used in mature cattle populations resident in northern Australia. DESIGN: Blood samples were collected from beef cattle in northern Queensland, the Northern Territory and northern Western Australia, and from dairy cattle in northern Queensland. The specificity of a serological test for JD was estimated by testing the blood samples with an absorbed ELISA kit. Further samples were collected from cattle with positive ELISA results to determine the presence or absence of infection with Mycobacterium avium subsp paratuberculosis. PROCEDURE: During 1995 and 1996, blood, tissue and gut contents were collected from beef cattle at abattoirs in Queensland and the Northern Territory; and blood and faecal samples were collected from dairy cattle in herds assessed to be most at risk for JD in northern Queensland. The blood samples were tested using an absorbed ELISA kit. Tissues and gut contents from beef cattle that had positive ELISA results were cultured for M. avium subsp paratuberculosis, and tissues were examined histologically. Faecal samples from dairy cattle with positive ELISA results were cultured for M. avium subsp paratuberculosis. RESULTS: Estimates of specificity for this absorbed ELISA in mature northern Australian cattle were 98.0% (97.0 to 98.8%, 95% CI) in beef cattle, and 98.3% (96.7 to 99.3%, 95% CI) in dairy cattle. CONCLUSION: Estimates of specificity in this study were lower for beef cattle from the Northern Territory and northern Western Australia and for dairy cattle from northern Queensland than those quoted from studies on cattle in southern Western Australia. This should be considered when serological testing using the JD ELISA is carried out on northern Australian cattle.
Authors: Michael T Collins; Scott J Wells; Kristine R Petrini; James E Collins; Ronald D Schultz; Robert H Whitlock Journal: Clin Diagn Lab Immunol Date: 2005-06
Authors: Kun Taek Park; Jongsam Ahn; William C Davis; Hye Cheong Koo; Nam Hoon Kwon; Woo Kyung Jung; Jun Man Kim; Soon Keun Hong; Yong Ho Park Journal: J Vet Sci Date: 2006-12 Impact factor: 1.672
Authors: Richard Whittington; Karsten Donat; Maarten F Weber; David Kelton; Søren Saxmose Nielsen; Suzanne Eisenberg; Norma Arrigoni; Ramon Juste; Jose Luis Sáez; Navneet Dhand; Annalisa Santi; Anita Michel; Herman Barkema; Petr Kralik; Polychronis Kostoulas; Lorna Citer; Frank Griffin; Rob Barwell; Maria Aparecida Scatamburlo Moreira; Iva Slana; Heike Koehler; Shoor Vir Singh; Han Sang Yoo; Gilberto Chávez-Gris; Amador Goodridge; Matjaz Ocepek; Joseba Garrido; Karen Stevenson; Mike Collins; Bernardo Alonso; Karina Cirone; Fernando Paolicchi; Lawrence Gavey; Md Tanvir Rahman; Emmanuelle de Marchin; Willem Van Praet; Cathy Bauman; Gilles Fecteau; Shawn McKenna; Miguel Salgado; Jorge Fernández-Silva; Radka Dziedzinska; Gustavo Echeverría; Jaana Seppänen; Virginie Thibault; Vala Fridriksdottir; Abdolah Derakhshandeh; Masoud Haghkhah; Luigi Ruocco; Satoko Kawaji; Eiichi Momotani; Cord Heuer; Solis Norton; Simeon Cadmus; Angelika Agdestein; Annette Kampen; Joanna Szteyn; Jenny Frössling; Ebba Schwan; George Caldow; Sam Strain; Mike Carter; Scott Wells; Musso Munyeme; Robert Wolf; Ratna Gurung; Cristobal Verdugo; Christine Fourichon; Takehisa Yamamoto; Sharada Thapaliya; Elena Di Labio; Monaya Ekgatat; Andres Gil; Alvaro Nuñez Alesandre; José Piaggio; Alejandra Suanes; Jacobus H de Waard Journal: BMC Vet Res Date: 2019-06-13 Impact factor: 2.741