K M Lemar1, M P Turner, D Lloyd. 1. Microbiology (BIOSI 1), Cardiff University, Cardiff, Wales, UK. Lemarkm@cf.ac.uk
Abstract
AIMS: To determine the effects of fresh and freeze-dried extracts of Allium sativum on the physiology and morphology of Candida albicans. METHODS AND RESULTS: Inhibition of growth in glucose-yeast extract-peptone was measured using a multiwell plate reader. Scanning and transmission electron microscopy investigations indicated loss of structural integrity. Gas chromatography-mass spectrometry of extracts was employed to separate and quantify putative inhibitory sulphur-containing components. CONCLUSIONS: Fresh garlic extract has a greater efficacy than garlic powder extract as indicated both by its effects on morphology and inhibition of growth. SIGNIFICANCE AND IMPACT OF THE STUDY: The ubiquitous opportunistic pathogen C. albicans is sensitive to garlic; resistance to the broad spectrum of active principles present is unlikely so that its anticandidal effects may provide an important alternative route to chemotherapy.
AIMS: To determine the effects of fresh and freeze-dried extracts of Allium sativum on the physiology and morphology of Candida albicans. METHODS AND RESULTS: Inhibition of growth in glucose-yeast extract-peptone was measured using a multiwell plate reader. Scanning and transmission electron microscopy investigations indicated loss of structural integrity. Gas chromatography-mass spectrometry of extracts was employed to separate and quantify putative inhibitory sulphur-containing components. CONCLUSIONS: Fresh garlic extract has a greater efficacy than garlic powder extract as indicated both by its effects on morphology and inhibition of growth. SIGNIFICANCE AND IMPACT OF THE STUDY: The ubiquitous opportunistic pathogen C. albicans is sensitive to garlic; resistance to the broad spectrum of active principles present is unlikely so that its anticandidal effects may provide an important alternative route to chemotherapy.
Authors: Katey M Lemar; Ourania Passa; Miguel A Aon; Sonia Cortassa; Carsten T Müller; Sue Plummer; Brian O'Rourke; David Lloyd Journal: Microbiology (Reading) Date: 2005-10 Impact factor: 2.777
Authors: Paulo César Venâncio; Sidney Raimundo Figueroba; Bruno Dias Nani; Luiz Eduardo Nunes Ferreira; Bruno Vilela Muniz; Fernando de Sá Del Fiol; Adilson Sartoratto; Edvaldo Antonio Ribeiro Rosa; Francisco Carlos Groppo Journal: Adv Pharm Bull Date: 2017-04-13