Literature DB >> 1214785

A simple method for the purification of reticulocyte globin messenger ribonucleic acid.

D Konecki, J M Cimadevilla, G Kramer, B Hardesty.   

Abstract

A relatively simple and inexpensive method has been developed for the preparation of highly purified rabbit reticulocyte globin mRNA. After phenol extraction, polysomal RNA was chromatographed on Sigmacell type 38 cellulose and Sepharose 4B. The resulting mRNA preparation has a purity in excess of 90%. No selective loss of either alpha or beta globin mRNA is observed.

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Year:  1975        PMID： 1214785     DOI： 10.1007/bf00357021

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  15 in total

1.  Globin mRNA translation on Artemia salina ribosomes with components from Friend leukemia cells.

Authors:  G A Kramer; P Pinphanichakarn; D Konecki; B A Hardesty
Journal:  Eur J Biochem       Date:  1975-05-06

2.  The binding of RNA to various celluloses.

Authors:  J DeLarco; G Guroff
Journal:  Biochem Biophys Res Commun       Date:  1973-01-23       Impact factor: 3.575

3.  Retention of ribosomal ribonucleic acids in agarose gels.

Authors:  S L Petrović; J S Petrović; M B Novaković
Journal:  Biochim Biophys Acta       Date:  1973-05-10

4.  Use of Sepharose 4B for preparative scale fractionation of eukaryotic messenger RNA's.

Authors:  S L Woo; S E Harris; J M Rosen; L Chan; P J Sperry; A R Means; B W O'Malley
Journal:  Prep Biochem       Date:  1974

5.  Characteristics of rabbit globin mRNA purification by oligo(dT) cellulose chromatography.

Authors:  J Gielen; H Aviv; P Leder
Journal:  Arch Biochem Biophys       Date:  1974-07       Impact factor: 4.013

6.  Isolation of eukaryotic messenger RNA on cellulose and its translation in vitro.

Authors:  G Schutz; M Beato; P Feigelson
Journal:  Biochem Biophys Res Commun       Date:  1972-11-01       Impact factor: 3.575

7.  Isolation of mRNA from KB-cells by affinity chromatography on polyuridylic acid covalently linked to Sepharose.

Authors:  U Lindberg; T Persson
Journal:  Eur J Biochem       Date:  1972-12-04

8.  An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA.

Authors:  J E Darnell; R Wall; R J Tushinski
Journal:  Proc Natl Acad Sci U S A       Date:  1971-06       Impact factor: 11.205

9.  A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells.

Authors:  S Y Lee; J Mendecki; G Brawerman
Journal:  Proc Natl Acad Sci U S A       Date:  1971-06       Impact factor: 11.205

10.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157
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