| Literature DB >> 12142439 |
Samuel Aparicio1, Jarrod Chapman, Elia Stupka, Nik Putnam, Jer-Ming Chia, Paramvir Dehal, Alan Christoffels, Sam Rash, Shawn Hoon, Arian Smit, Maarten D Sollewijn Gelpke, Jared Roach, Tania Oh, Isaac Y Ho, Marie Wong, Chris Detter, Frans Verhoef, Paul Predki, Alice Tay, Susan Lucas, Paul Richardson, Sarah F Smith, Melody S Clark, Yvonne J K Edwards, Norman Doggett, Andrey Zharkikh, Sean V Tavtigian, Dmitry Pruss, Mary Barnstead, Cheryl Evans, Holly Baden, Justin Powell, Gustavo Glusman, Lee Rowen, Leroy Hood, Y H Tan, Greg Elgar, Trevor Hawkins, Byrappa Venkatesh, Daniel Rokhsar, Sydney Brenner.
Abstract
The compact genome of Fugu rubripes has been sequenced to over 95% coverage, and more than 80% of the assembly is in multigene-sized scaffolds. In this 365-megabase vertebrate genome, repetitive DNA accounts for less than one-sixth of the sequence, and gene loci occupy about one-third of the genome. As with the human genome, gene loci are not evenly distributed, but are clustered into sparse and dense regions. Some "giant" genes were observed that had average coding sequence sizes but were spread over genomic lengths significantly larger than those of their human orthologs. Although three-quarters of predicted human proteins have a strong match to Fugu, approximately a quarter of the human proteins had highly diverged from or had no pufferfish homologs, highlighting the extent of protein evolution in the 450 million years since teleosts and mammals diverged. Conserved linkages between Fugu and human genes indicate the preservation of chromosomal segments from the common vertebrate ancestor, but with considerable scrambling of gene order.Entities:
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Year: 2002 PMID： 12142439 DOI： 10.1126/science.1072104
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728