| Literature DB >> 12141527 |
U Hladnik1, L Braida, M Boniotto, D Pirulli, F Gerin, A Amoroso, S Crovella.
Abstract
Recently several authors correlated MBL-2 gene polymorphisms with different pathologies and there is a growing interest for MBL-2 genotyping in a large number of individuals. We have developed a single-tube, rapid, economic, and fully automated melting temperature analysis screening method, based on ABI 7700 Sequence Detection System technology and SYBR Green I chemistry, for the detection of three polymorphisms (exon 1, codons 52, 54, 57) in the MBL-2 gene. We also developed an electronic sheet for the automatic calling of different genotypes, based on the analysis of the first derivative of ABI 7700 raw data.Entities:
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Year: 2002 PMID： 12141527 DOI： 10.1007/s102380200014
Source DB: PubMed Journal: Clin Exp Med ISSN： 1591-8890 Impact factor: 3.984