Literature DB >> 12124933

Establishing two-dimensional gels for the analysis of Leishmania proteomes.

Nathalie Acestor1, Slavica Masina, John Walker, Nancy G Saravia, Nicolas Fasel, Manfredo Quadroni.   

Abstract

Several different sample preparation methods for two-dimensional electrophoresis (2-DE) analysis of Leishmania parasites were compared. From this work, we were able to identify a solubilization method using Nonidet P-40 as detergent, which was simple to follow, and which produced 2-DE gels of high resolution and reproducibility.

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Year:  2002        PMID: 12124933     DOI: 10.1002/1615-9861(200207)2:7<877::AID-PROT877>3.0.CO;2-D

Source DB:  PubMed          Journal:  Proteomics        ISSN: 1615-9853            Impact factor:   3.984


  6 in total

1.  Identification of osteocyte-selective proteins.

Authors:  Dayong Guo; Andrew Keightley; Jill Guthrie; Patricia A Veno; Stephen E Harris; Lynda F Bonewald
Journal:  Proteomics       Date:  2010-10       Impact factor: 3.984

2.  Comparative Two-dimensional Gel Electrophoresis Maps for Amastigote-like Proteomes of Iranian Leishmania Tropica and Leishmania Major Isolates.

Authors:  Marzieh Ashrafmansouri; Fatemeh Sadat Sadjjadi; Seyyedjavad Seyyedtabaei; Ali Haghighi; Mostafa Rezaei-Tavirani; Nayebali Ahmadi
Journal:  Galen Med J       Date:  2019-08-15

3.  Comparison of Proteome Profiling of Two Sensitive and Resistant Field Iranian Isolates of Leishmania major to Glucantime® by 2- Dimensional Electrophoresis.

Authors:  Mehdi Zarean; Sharif Maraghi; Homa Hajjaran; Mehdi Mohebali; Mohammad Hossein Feiz-Hadad; Mohammad Ali Assarehzadegan
Journal:  Iran J Parasitol       Date:  2015 Jan-Mar       Impact factor: 1.012

4.  Quantitative proteomic analysis of amastigotes from Leishmania (L.) amazonensis LV79 and PH8 strains reveals molecular traits associated with the virulence phenotype.

Authors:  Eloiza de Rezende; Rebeca Kawahara; Mauricio S Peña; Giuseppe Palmisano; Beatriz S Stolf
Journal:  PLoS Negl Trop Dis       Date:  2017-11-27

5.  Plants with anti-Leishmania activity: Integrative review from 2000 to 2011.

Authors:  Ana Maria G Brito; Derivaldo Dos Santos; Sheyla A Rodrigues; Renan G Brito; Lauro Xavier-Filho
Journal:  Pharmacogn Rev       Date:  2013-01

Review 6.  Using Proteomics to Understand How Leishmania Parasites Survive inside the Host and Establish Infection.

Authors:  Patrícia Sampaio Tavares Veras; Juliana Perrone Bezerra de Menezes
Journal:  Int J Mol Sci       Date:  2016-08-19       Impact factor: 5.923

  6 in total

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