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Literature DB >> 1212222

Purification and characterization of guinea-pig intestinal brush borders.

K Andersen, G V Lippe, L Morkrid, H Schjonsby.

Abstract

The purification of guinea-pig intestinal brush borders by a rapid sucrose-gradient-centrifugation step is reported. A 29-fold increase in the maltase/DNA quotient indicates considerable purification of the brush borders from nuclei. The biological activity of the brush borders was well preserved, as demonstrated by a high recovery of human gastric-juice-mediated uptake of 57Co-labelled vitamin B-12; homogeneity and purity were confirmed by scanning electron microscopy. Both the morphological appearance and biological activity were unchanged after prolonged storage in glycerol.

Entities: Chemical Species

Mesh：

Substances：
DNA

Year: 1975 PMID： 1212222 PMCID： PMC1172451 DOI： 10.1042/bj1520157

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

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References

14 in total

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Authors: K S GOTTLIEBLAU; L R WASSERMAN; V HERBERT
Journal: Blood Date: 1965-06 Impact factor: 22.113

Purification and characterization of guinea-pig intestinal brush borders.

1. The isolation of purified brush borders from rat small intestine.

2. [ON AMINO ACID ARYLAMIDASE (SO-CALLED LEUCINE AMINOPEPTIDASE) ACTIVITY IN THE HUMAN SERUM].

3. A procedure for the isolation of the epithelial brush border membrane of hamster small intestine.

4. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

5. The digestive function of the epithelium of the small intestine. II. Localization of disaccharide hydrolysis in the isolated brush border portion of intestinal epithelial cells.

6. Studies on the fractionation of mucosal homogenates from the small intestine.

7. Assay of intestinal disaccharidases.

8. Intrinsic factor-mediated attachment of vitamin B12 to brush borders and microvillous membranes of hamster intestine.

9. THE ISOLATION AND CHARACTERIZATION OF SUBCELLULAR COMPONENTS OF THE EPITHELIAL CELLS OF RABBIT SMALL INTESTINE.

10. RAPID CHARCOAL ASSAY FOR INTRINSIC FACTOR (IF), GASTRIC JUICE UNSATURATED B12 BINDING CAPACITY, ANTIBODY TO IF, AND SERUM UNSATURATED B12 BINDING CAPACITY.