Literature DB >> 12093699

Microimmunoassay using a protein chip: optimizing conditions for protein immobilization.

Seung-Yong Seong1.   

Abstract

Optimizing conditions for the microarraying of protein antigens onto glass slides were studied. Various vendors, surface functional groups, buffers, and fixatives were evaluated to enhance protein binding. A total of 125 pg of human immunoglobulin was detectable with this assay system, suggesting that protein microarray can be applied for routine immunodiagnosis.

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Year:  2002        PMID： 12093699      PMCID： PMC120026          DOI： 10.1128/cdli.9.4.927-930.2002

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  15 in total

Review 1.  Large-scale functional analysis using peptide or protein arrays.

Authors:  A Q Emili; G Cagney
Journal:  Nat Biotechnol       Date:  2000-04       Impact factor: 54.908

2.  Protein microchips: use for immunoassay and enzymatic reactions.

Authors:  P Arenkov; A Kukhtin; A Gemmell; S Voloshchuk; V Chupeeva; A Mirzabekov
Journal:  Anal Biochem       Date:  2000-02-15       Impact factor: 3.365

3.  Chip-based mosaic immunoassays.

Authors:  S Katzman
Journal:  Anal Chem       Date:  2001-01-01       Impact factor: 6.986

4.  Printing proteins as microarrays for high-throughput function determination.

Authors:  G MacBeath; S L Schreiber
Journal:  Science       Date:  2000-09-08       Impact factor: 47.728

5.  Patterning of functional antibodies and other proteins by photolithography of silane monolayers.

Authors:  J F Mooney; A J Hunt; J R McIntosh; C A Liberko; D M Walba; C T Rogers
Journal:  Proc Natl Acad Sci U S A       Date:  1996-10-29       Impact factor: 11.205

Review 6.  Criticality found in a model for orientational ordering of protein arrays.

Authors:  M Hogyoku
Journal:  Adv Biophys       Date:  1997

7.  Immobilization of streptavidin for immunosensors on nanostructured surfaces.

Authors:  S Kossek; C Padeste; L Tiefenauer
Journal:  J Mol Recognit       Date:  1996 Sep-Dec       Impact factor: 2.137

8.  Layered expression scanning: rapid molecular profiling of tumor samples.

Authors:  C R Englert; G V Baibakov; M R Emmert-Buck
Journal:  Cancer Res       Date:  2000-03-15       Impact factor: 12.701

9.  Fabrication of microarray of gel-immobilized compounds on a chip by copolymerization.

Authors:  A V Vasiliskov; E N Timofeev; S A Surzhikov; A L Drobyshev; V V Shick; A D Mirzabekov
Journal:  Biotechniques       Date:  1999-09       Impact factor: 1.993

10.  Oligonucleotide-directed self-assembly of proteins: semisynthetic DNA--streptavidin hybrid molecules as connectors for the generation of macroscopic arrays and the construction of supramolecular bioconjugates.

Authors:  C M Niemeyer; T Sano; C L Smith; C R Cantor
Journal:  Nucleic Acids Res       Date:  1994-12-25       Impact factor: 16.971
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  2 in total

1.  Multiplexed immunosensing and kinetics monitoring in nanofluidic devices with highly enhanced target capture efficiency.

Authors:  Yii-Lih Lin; Yen-Jun Huang; Pattamon Teerapanich; Thierry Leïchlé; Chia-Fu Chou
Journal:  Biomicrofluidics       Date:  2016-06-07       Impact factor: 2.800

2.  Yeast surface display-based microfluidic immunoassay.

Authors:  Jing Wang; Danhui Cheng; Jay Kwok-Lun Chan; Xiaoteng Luo; Hongkai Wu; I-Ming Hsing
Journal:  Sens Actuators B Chem       Date:  2012-03-07       Impact factor: 7.460
  2 in total

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