Literature DB >> 12059112

Modification of fatty acid composition in tomato (Lycopersicon esculentum) by expression of a borage delta6-desaturase.

David Cook1, Don Grierson, Craigh Jones, Andrew Wallace, Gill West, Greg Tucker.   

Abstract

The improvement of nutritional quality is one potential application for the genetic modification of plants. One possible target for such manipulation is the modification of fatty acid metabolism. In this work, expression of a borage delta6-desaturase cDNA in tomato (Lycopersicon esculentum L.) has been shown to produce gamma-linolenic acid (GLA; 18:83 delta6,9,12) and octadecatetraenoic acid (OTA; 18:4 delta6,9,12,15) in transgenic leaf and fruit tissue. This genetic modification has also, unexpectedly, resulted in a reduction in the percentage of linoleic acid (LA 18:2 delta9,12) and a concomitant increase in the percentage of alpha-linolenic acid (ALA; 18:3 delta9,12,15) in fruit tissue. These changes in fatty acid composition are thought to be beneficial for human health.

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Year:  2002        PMID： 12059112     DOI： 10.1385/MB:21:2:123

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.860


  8 in total

Review 1.  Plant desaturases: harvesting the fat of the land.

Authors:  J A Napier; L V Michaelson; A K Stobart
Journal:  Curr Opin Plant Biol       Date:  1999-04       Impact factor: 7.834

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Journal:  Plant Cell       Date:  1989-03       Impact factor: 11.277

3.  Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of delta6-desaturated fatty acids in transgenic tobacco.

Authors:  O Sayanova; M A Smith; P Lapinskas; A K Stobart; G Dobson; W W Christie; P R Shewry; J A Napier
Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-15       Impact factor: 11.205

Review 4.  Nutritional aspects of cardiovascular disease. Report of the Cardiovascular Review Group Committee on Medical Aspects of Food Policy.

Authors: 
Journal:  Rep Health Soc Subj (Lond)       Date:  1994

5.  Isolation of a delta 6-desaturase gene from the cyanobacterium Synechocystis sp. strain PCC 6803 by gain-of-function expression in Anabaena sp. strain PCC 7120.

Authors:  A S Reddy; M L Nuccio; L M Gross; T L Thomas
Journal:  Plant Mol Biol       Date:  1993-05       Impact factor: 4.076

6.  Plants as 'chemical factories' for the production of polyunsaturated fatty acids.

Authors:  D L Alonso; F G Maroto
Journal:  Biotechnol Adv       Date:  2000-10       Impact factor: 14.227

7.  Expression of a cyanobacterial delta 6-desaturase gene results in gamma-linolenic acid production in transgenic plants.

Authors:  A S Reddy; T L Thomas
Journal:  Nat Biotechnol       Date:  1996-05       Impact factor: 54.908

8.  Histidine-41 of the cytochrome b5 domain of the borage delta6 fatty acid desaturase is essential for enzyme activity.

Authors:  O Sayanova; P R Shewry; J A Napier
Journal:  Plant Physiol       Date:  1999-10       Impact factor: 8.340
  8 in total
  2 in total

1.  Network Analysis Provides Insight into Tomato Lipid Metabolism.

Authors:  Anastasiya Kuhalskaya; Micha Wijesingha Ahchige; Leonardo Perez de Souza; José Vallarino; Yariv Brotman; Saleh Alseekh
Journal:  Metabolites       Date:  2020-04-14

Review 2.  Plants as bioreactors: Recent developments and emerging opportunities.

Authors:  Arun K Sharma; Manoj K Sharma
Journal:  Biotechnol Adv       Date:  2009-06-30       Impact factor: 14.227
  2 in total

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