BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha), may be involved in the pathogenesis of inflammatory bowel diseases (IBDs). The aim of this study was to evaluate the effect of TNF-alpha on the inflammatory activity of IBD. METHODS: TNF-alpha mRNA expression in intestinal mucosal biopsy specimens from IBD patients [ulcerative colitis (UC), n = 54; and Crohn's disease (CD), n = 11] was analyzed using a competitive polymerase chain reaction. The degree of macrophage infiltration was analyzed by immunohistochemistry, using an antihuman CD68 antibody. RESULTS: TNF-alpha mRNA expression was increased in UC patients, corresponding to the inflammatory activity. However, in CD, TNF-alpha mRNA expression was not correlated with the endoscopic findings. CONCLUSIONS: We clarified that TNF-alpha mRNA expression was responsible for the inflammatory activity in UC. However, TNF-alpha mRNA expression was not correlated with the mucosal injury in CD.
BACKGROUND:Tumor necrosis factor-alpha (TNF-alpha), may be involved in the pathogenesis of inflammatory bowel diseases (IBDs). The aim of this study was to evaluate the effect of TNF-alpha on the inflammatory activity of IBD. METHODS:TNF-alpha mRNA expression in intestinal mucosal biopsy specimens from IBDpatients [ulcerative colitis (UC), n = 54; and Crohn's disease (CD), n = 11] was analyzed using a competitive polymerase chain reaction. The degree of macrophage infiltration was analyzed by immunohistochemistry, using an antihuman CD68 antibody. RESULTS:TNF-alpha mRNA expression was increased in UC patients, corresponding to the inflammatory activity. However, in CD, TNF-alpha mRNA expression was not correlated with the endoscopic findings. CONCLUSIONS: We clarified that TNF-alpha mRNA expression was responsible for the inflammatory activity in UC. However, TNF-alpha mRNA expression was not correlated with the mucosal injury in CD.
Authors: B S Boland; D L Boyle; W J Sandborn; G S Firestein; B G Levesque; J Hillman; B Zhang; J Proudfoot; L Eckmann; P B Ernst; J Rivera-Nieves; S Pola; N Copur-Dahi; J T Chang Journal: Aliment Pharmacol Ther Date: 2014-07-13 Impact factor: 8.171
Authors: Agnes Vatay; László Bene; Agota Kovács; Zoltán Prohászka; Csaba Szalai; László Romics; Béla Fekete; István Karádi; George Füst Journal: Immunogenetics Date: 2003-06-14 Impact factor: 2.846
Authors: Alberto J León; Emma Gómez; Jose A Garrote; David Bernardo; Asterio Barrera; Jose L Marcos; Luis Fernández-Salazar; Benito Velayos; Alfredo Blanco-Quirós; Eduardo Arranz Journal: Mediators Inflamm Date: 2009-07-30 Impact factor: 4.711