OBJECTIVE: Elevations in cell free fetal DNA has previously been determined in pregnancies affected by preeclampsia. A recent report has indicated that cell free fetal DNA concentrations are elevated early in pregnancy before disease onset. As we have recently performed a prospective study to examine fetal cell traffic in pregnancies at risk for developing preeclampsia, we now quantify cell free fetal DNA concentrations in these samples. METHODS: Blood samples were collected in the second trimester of pregnancy from pregnancies at risk for preeclampsia. Cell free fetal DNA amounts were quantified by real-time PCR. These results were then correlated with subsequent pregnancy outcome. RESULTS: Free fetal DNA levels were significantly higher (median of 422.9 vs. 128.5 copies/mL maternal plasma; p=0.005) in plasma samples from women who developed preeclampsia ( n=10) when compared to those who had unremarkable pregnancies ( n=40). CONCLUSIONS: Our data independently confirm the finding that maternal plasma cell free fetal DNA levels are elevated early in pregnancies, which later develop preeclampsia.
OBJECTIVE: Elevations in cell free fetal DNA has previously been determined in pregnancies affected by preeclampsia. A recent report has indicated that cell free fetal DNA concentrations are elevated early in pregnancy before disease onset. As we have recently performed a prospective study to examine fetal cell traffic in pregnancies at risk for developing preeclampsia, we now quantify cell free fetal DNA concentrations in these samples. METHODS: Blood samples were collected in the second trimester of pregnancy from pregnancies at risk for preeclampsia. Cell free fetal DNA amounts were quantified by real-time PCR. These results were then correlated with subsequent pregnancy outcome. RESULTS: Free fetal DNA levels were significantly higher (median of 422.9 vs. 128.5 copies/mL maternal plasma; p=0.005) in plasma samples from women who developed preeclampsia ( n=10) when compared to those who had unremarkable pregnancies ( n=40). CONCLUSIONS: Our data independently confirm the finding that maternal plasma cell free fetal DNA levels are elevated early in pregnancies, which later develop preeclampsia.
Authors: Robert M Silver; Leslie Myatt; John C Hauth; Kenneth J Leveno; Alan M Peaceman; Susan M Ramin; Philip Samuels; George Saade; Yoram Sorokin; Rebecca G Clifton; Uma M Reddy Journal: Am J Perinatol Date: 2016-07-11 Impact factor: 1.862
Authors: Aaron F Orozco; Carolina J Jorgez; Cassandra Horne; Deborah A Marquez-Do; Matthew R Chapman; John R Rodgers; Farideh Z Bischoff; Dorothy E Lewis Journal: Am J Pathol Date: 2008-10-30 Impact factor: 4.307
Authors: A F Orozco; C J Jorgez; W D Ramos-Perez; E J Popek; X Yu; C A Kozinetz; F Z Bischoff; D E Lewis Journal: Placenta Date: 2009-08-18 Impact factor: 3.481