Literature DB >> 12036488

Construction and characterization of an infectious molecular clone derived from the CRF01_AE primary isolate of HIV type 1.

Tetsu Mukai1, Satoshi Komoto, Takeshi Kurosu, J Alejandro Palacios, Yong-Gang Li, Wattana Auwanit, Masashi Tatsumi, Kazuyoshi Ikuta.   

Abstract

An infectious molecular clone (named p95TNIH022) was constructed using long-range polymerase chain reaction products derived from a clinical isolate (95TNIH022) of HIV-1 CRF01_AE obtained from an asymptomatic Thai carrier in 1995. The virus in the supernatant from p95TNIH022-transfected 293T cells showed infectivity in peripheral blood mononuclear cells (PBMCs) as well as in MAGIC5 cells, which express CD4 and CCR5, but not in the original MAGI cells, indicating that p95TNIH022 is an infectious molecular clone with CCR5 tropism. Interestingly, p95TNIH022-derived virus induced profound cell killing in infected PBMCs, as in cells infected with the parental isolate.

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Year:  2002        PMID： 12036488     DOI： 10.1089/088922202753747932

Source DB:  PubMed          Journal:  AIDS Res Hum Retroviruses        ISSN： 0889-2229            Impact factor:   2.205


  5 in total

1.  Infectious molecular clone of a recently transmitted pediatric human immunodeficiency virus clade C isolate from Africa: evidence of intraclade recombination.

Authors:  Ricky D Grisson; Agnès-Laurence Chenine; Lan-Yu Yeh; Jun He; Charles Wood; Ganapati J Bhat; Weidong Xu; Chipepo Kankasa; Ruth M Ruprecht
Journal:  J Virol       Date:  2004-12       Impact factor: 5.103

2.  Parental LTRs are important in a construct of a stable and efficient replication-competent infectious molecular clone of HIV-1 CRF08_BC.

Authors:  Qiwei Zhang; Xiaomin Zhang; Hao Wu; Donald Seto; Hao-Jie Zhang; Zhiwei Chen; Chengsong Wan; Bo-Jian Zheng
Journal:  PLoS One       Date:  2012-02-17       Impact factor: 3.240

3.  L565M mutation in HIV-1 glycoprotein 41 stabilizes the coiled-coil structure.

Authors:  Daisuke Yamamoto; Gui-Mei Li; Kazuyoshi Ikuta; Toshiyuki Goto
Journal:  Biochem Biophys Res Commun       Date:  2005-09-16       Impact factor: 3.575

4.  Isolation and characterization of a replication-competent molecular clone of an HIV-1 circulating recombinant form (CRF33_01B).

Authors:  Kok Keng Tee; Shigeru Kusagawa; Xiao-Jie Li; Narumi Onogi; Maya Isogai; Saiki Hase; Rie Uenishi; Huanan Liao; Adeeba Kamarulzaman; Yutaka Takebe
Journal:  PLoS One       Date:  2009-08-18       Impact factor: 3.240

5.  Construction and characterization of highly infectious full-length molecular clones of a HIV-1 CRF07_BC isolate from Xinjiang, China.

Authors:  Zheng Wang; Kunxue Hong; Jing Zhang; Lei Zhang; Dan Li; Li Ren; Hua Liang; Yiming Shao
Journal:  PLoS One       Date:  2013-11-18       Impact factor: 3.240
  5 in total

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