PURPOSE: We compared three different probe combinations for detection of postzygotic mosaic imbalances in human preimplantation embryos. METHODS: Two hundred and two spare cleavage stage embryos were hybridized with fluorescently labelled DNA probe mixtures specific to chromosomes X, Y, 18 (N = 67), chromosomes 2, 7, 18 (N = 71), or chromosomes 13, 16, 18, 21, 22 (N = 64). RESULTS: An overall higher incidence of abnormalities was detected using probe mixture for five (69%) or three (72%) autosomes compared to one autosome and chromosomes X and Y (54%). The rate of aneuploidy detected increased with the number of autosomes hybridized from 4% (X, Y, 18) to 11% (2, 7, 18) to 19% (13, 16, 18, 21, 22). Postzygotic mosaicism comprised the most frequent abnormality detected by all probe combinations, and the percentage detected by each was similar, 48% (X, Y, 18), 56% (2, 7,18), and 50% (13,16,18, 21, 22). CONCLUSIONS: A probe combination of five autosomes, particularly those of clinical relevance, may be more beneficial for screening embryos from patients at risk of maternal-age-related aneuploidy. However, all three probe combinations are as efficient at identifying postzygotic mosaicism, and may be used for identifying embryos with less potential of developing to term.
PURPOSE: We compared three different probe combinations for detection of postzygotic mosaic imbalances in human preimplantation embryos. METHODS: Two hundred and two spare cleavage stage embryos were hybridized with fluorescently labelled DNA probe mixtures specific to chromosomes X, Y, 18 (N = 67), chromosomes 2, 7, 18 (N = 71), or chromosomes 13, 16, 18, 21, 22 (N = 64). RESULTS: An overall higher incidence of abnormalities was detected using probe mixture for five (69%) or three (72%) autosomes compared to one autosome and chromosomes X and Y (54%). The rate of aneuploidy detected increased with the number of autosomes hybridized from 4% (X, Y, 18) to 11% (2, 7, 18) to 19% (13, 16, 18, 21, 22). Postzygotic mosaicism comprised the most frequent abnormality detected by all probe combinations, and the percentage detected by each was similar, 48% (X, Y, 18), 56% (2, 7,18), and 50% (13,16,18, 21, 22). CONCLUSIONS: A probe combination of five autosomes, particularly those of clinical relevance, may be more beneficial for screening embryos from patients at risk of maternal-age-related aneuploidy. However, all three probe combinations are as efficient at identifying postzygotic mosaicism, and may be used for identifying embryos with less potential of developing to term.
Authors: Y Sasabe; K P Katayama; T Nishimura; A Takahashi; H Asakura; K Winchester-Peden; L Wise; Y Abe; H Kubo; S Hirakawa Journal: J Assist Reprod Genet Date: 1999-02 Impact factor: 3.412
Authors: C Staessen; E Van Assche; H Joris; M Bonduelle; M Vandervorst; I Liebaers; A Van Steirteghem Journal: Mol Hum Reprod Date: 1999-04 Impact factor: 4.025
Authors: Y Verlinsky; J Cieslak; V Ivakhnenko; S Evsikov; G Wolf; M White; A Lifchez; B Kaplan; J Moise; J Valle; N Ginsberg; C Strom; A Kuliev Journal: J Assist Reprod Genet Date: 1999-04 Impact factor: 3.412
Authors: Victoria Burruel; Katie Klooster; Christopher M Barker; Renee Reijo Pera; Stuart Meyers Journal: Sci Rep Date: 2014-10-13 Impact factor: 4.379