Literature DB >> 1200740

Oxidation of formaldehyde by alcohol oxidase of Candida boidinii.

H Sahm.   

Abstract

A fromaldehyde oxidase activity was found in cellfree extracts of methanol-grown yeast Candida boidinii. Loss of alcohol oxidase activity in a mutant, 48, led to loss of the formaldehyde oxidase activity, indicating that the same enzyme is probably responsible for both activities. This could be demonstrated with the purified alcohol oxidase which oxidizes, besides lower primary alcohols, formaldehyde to formate. The Km value for formaldehyde is 5.7 mM. It seems that alcohol oxidase is not implicated in formaldehyde oxidation in vivo.

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Year:  1975        PMID： 1200740     DOI： 10.1007/bf00447134

Source DB:  PubMed          Journal:  Arch Microbiol        ISSN： 0302-8933            Impact factor:   2.552


  8 in total

1.  The dismutation of formaldehyde by liver alcohol dehydrogenase.

Authors:  R H ABELES; H A LEE
Journal:  J Biol Chem       Date:  1960-05       Impact factor: 5.157

2.  The colorimetric estimation of formaldehyde by means of the Hantzsch reaction.

Authors:  T NASH
Journal:  Biochem J       Date:  1953-10       Impact factor: 3.857

3.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

4.  Microbial assimilation of methanol. The ethanol- and methanol-oxidizing enzymes of the yeast Candida boidinii.

Authors:  H Sahm; F Wagner
Journal:  Eur J Biochem       Date:  1973-07-02

5.  [Microbial assimilation of methanol. Properties of formaldehyde dehydrogenase and formate dehydrogenase from Candida boidinii].

Authors:  H Sahm; F Wagner
Journal:  Arch Mikrobiol       Date:  1973

6.  [Microbial assimilation of methanol. Isolation and characterization of the yeast Candida boidinii].

Authors:  H Sahm; F Wagner
Journal:  Arch Mikrobiol       Date:  1972

7.  Physiological studies of methane- and methanol-oxidizing bacteria: comparison of a primary alcohol dehydrogenase from Methylococcus capsulatus (Texas strain) and Pseudomonas species M27.

Authors:  R N Patel; H R Bose; W J Mandy; D S Hoare
Journal:  J Bacteriol       Date:  1972-05       Impact factor: 3.490

8.  Substrate specificity of the purified primary alcohol dehydrogenases from methanol-oxidizing bacteria.

Authors:  G T Sperl; H S Forrest; D T Gibson
Journal:  J Bacteriol       Date:  1974-05       Impact factor: 3.490
  8 in total
  4 in total

1.  Oxalate, formate, formamide, and methanol metabolism in Thiobacillus novellus.

Authors:  T S Chandra; Y I Shethna
Journal:  J Bacteriol       Date:  1977-08       Impact factor: 3.490

2.  S-formylgluthathione: the substrate for formate dehydrogenase in methanol-utilizing yeasts.

Authors:  J P van Dijken; G J Oostra-Demkes; R Otto; W Harder
Journal:  Arch Microbiol       Date:  1976-12-01       Impact factor: 2.552

3.  Methanol metabolism in a peroxisome-deficient mutant of Hansenula polymorpha: a physiological study.

Authors:  I J van der Klei; W Harder; M Veenhuis
Journal:  Arch Microbiol       Date:  1991       Impact factor: 2.552

4.  Purification and properties of S-hydroxymethylglutathione dehydrogenase of Paecilomyces variotii no. 5, a formaldehyde-degrading fungus.

Authors:  Ryohei Fukuda; Kazuhiro Nagahama; Kohsai Fukuda; Keisuke Ekino; Takuji Oka; Yoshiyuki Nomura
Journal:  AMB Express       Date:  2012-06-25       Impact factor: 3.298
  4 in total

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