Literature DB >> 12005075

A simple method for enrichment of uninucleate conidia of Aspergillus oryzae.

Seiichi Hara1, Ryohei F Tsuji, Osamu Hatamoto, Tsutomu Masuda.   

Abstract

Aspergillus oryzae produces multinucleate conidia, which makes the obtaining of homokaryons labor-intensive. Analysis of conidia by flow cytometry clarified the relationship that conidia of lower nuclear number were smaller in size. Based on this, we have developed a simple way to enrich uninucleate conidia with a membrane filter. Our results also suggest that the method is useful for elimination of heterokaryons.

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Year:  2002        PMID: 12005075     DOI: 10.1271/bbb.66.693

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN: 0916-8451            Impact factor:   2.043


  3 in total

1.  A further study on chromosome minimization by protoplast fusion in Aspergillus oryzae.

Authors:  Seiichi Hara; Feng Jie Jin; Tadashi Takahashi; Yasuji Koyama
Journal:  Mol Genet Genomics       Date:  2011-12-30       Impact factor: 3.291

2.  Novel hydrophobic surface binding protein, HsbA, produced by Aspergillus oryzae.

Authors:  Shinsaku Ohtaki; Hiroshi Maeda; Toru Takahashi; Youhei Yamagata; Fumihiko Hasegawa; Katsuya Gomi; Tasuku Nakajima; Keietsu Abe
Journal:  Appl Environ Microbiol       Date:  2006-04       Impact factor: 4.792

3.  A Special Phenotype of Aconidial Aspergillus niger SH2 and Its Mechanism of Formation via CRISPRi.

Authors:  Le-Yi Yu; Lin-Xiang Li; Lin-Lin Yao; Jun-Wei Zheng; Bin Wang; Li Pan
Journal:  J Fungi (Basel)       Date:  2022-06-28
  3 in total

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