Literature DB >> 11990994

Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode solid-phase extraction .

Petre Ivanov Dobrev1, Miroslav Kamínek.   

Abstract

A method for separation of cytokinins from auxin and abscisic acid, which allows further separation of cytokinin ribotides from cytokinin bases, ribosides and glucosides and their purification on a single Oasis MCX column was developed. Due to the mixed reversed-phase and cation-exchange mode of the Oasis MCX sorbent the cationic cytokinin bases, ribosides and glucosides as well as the anionic auxin, abscisic acid and cytokinin ribotides are retained and can be sequentially eluted by solvents containing different concentrations of methanol and ammonium hydroxide. Characteristics of the method are high recoveries of analyzed phytohormones and their sufficient purity for quantification by HPLC-ELISA (RIA) or HPLC-MS.

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Year:  2002        PMID： 11990994     DOI： 10.1016/s0021-9673(02)00024-9

Source DB:  PubMed          Journal:  J Chromatogr A        ISSN： 0021-9673            Impact factor:   4.759


  135 in total

1.  Cold response of dedifferentiated barley cells at the gene expression, hormone composition, and freezing tolerance levels: studies on callus cultures.

Authors:  Ildikó Vashegyi; Zsuzsa Marozsán-Tóth; Gábor Galiba; Petre I Dobrev; Radomira Vankova; Balázs Tóth
Journal:  Mol Biotechnol       Date:  2013-06       Impact factor: 2.695

2.  Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro.

Authors:  Branka Uzelac; Dušica Janošević; Ana Simonović; Václav Motyka; Petre I Dobrev; Snežana Budimir
Journal:  Protoplasma       Date:  2015-04-03       Impact factor: 3.356

3.  Expression of a transcription factor from Capsicum annuum in pine calli counteracts the inhibitory effects of salt stress on adventitious shoot formation.

Authors:  Wei Tang; Ronald J Newton; Jinxing Lin; Thomas M Charles
Journal:  Mol Genet Genomics       Date:  2006-06-10       Impact factor: 3.291

4.  Subcellular homeostasis of phytohormone auxin is mediated by the ER-localized PIN5 transporter.

Authors:  Jozef Mravec; Petr Skůpa; Aurélien Bailly; Klára Hoyerová; Pavel Krecek; Agnieszka Bielach; Jan Petrásek; Jing Zhang; Vassilena Gaykova; York-Dieter Stierhof; Petre I Dobrev; Katerina Schwarzerová; Jakub Rolcík; Daniela Seifertová; Christian Luschnig; Eva Benková; Eva Zazímalová; Markus Geisler; Jirí Friml
Journal:  Nature       Date:  2009-06-07       Impact factor: 49.962

5.  Reproductive organs regulate leaf nitrogen metabolism mediated by cytokinin signal.

Authors:  Daisuke Igarashi; Yoshihiro Izumi; Yuko Dokiya; Kazuhiko Totsuka; Eiichiro Fukusaki; Chieko Ohsumi
Journal:  Planta       Date:  2008-12-02       Impact factor: 4.116

6.  Cell-Type-Specific Cytokinin Distribution within the Arabidopsis Primary Root Apex.

Authors:  Ioanna Antoniadi; Lenka Plačková; Biljana Simonovik; Karel Doležal; Colin Turnbull; Karin Ljung; Ondřej Novák
Journal:  Plant Cell       Date:  2015-07-07       Impact factor: 11.277

7.  Consequences of transforming narrow leafed lupin (Lupinus angustifolius [L.]) with an ipt gene under control of a flower-specific promoter.

Authors:  Craig A Atkins; R J Neil Emery; Penelope M C Smith
Journal:  Transgenic Res       Date:  2011-02-23       Impact factor: 2.788

8.  Cytokinin-induced VvTFL1A expression may be involved in the control of grapevine fruitfulness.

Authors:  Omer Crane; Tamar Halaly; Xuequn Pang; Shimon Lavee; Avi Perl; Radomira Vankova; Etti Or
Journal:  Planta       Date:  2011-08-24       Impact factor: 4.116

9.  A method for separation and determination of cytokinin nucleotides from plant tissues.

Authors:  Kentaro Takei; Tomoyuki Yamaya; Hitoshi Sakakibara
Journal:  J Plant Res       Date:  2003-05-07       Impact factor: 2.629

10.  Leptosphaeria maculans effector AvrLm4-7 affects salicylic acid (SA) and ethylene (ET) signalling and hydrogen peroxide (H2 O2 ) accumulation in Brassica napus.

Authors:  Miroslava Nováková; Vladimír Šašek; Lucie Trdá; Hana Krutinová; Thomas Mongin; Olga Valentová; Marie-HelEne Balesdent; Thierry Rouxel; Lenka Burketová
Journal:  Mol Plant Pathol       Date:  2016-01-06       Impact factor: 5.663
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