Literature DB >> 12734720

A method for separation and determination of cytokinin nucleotides from plant tissues.

Kentaro Takei1, Tomoyuki Yamaya, Hitoshi Sakakibara.   

Abstract

Recent progress in the study of cytokinin metabolism in plants indicates that quantitative analysis of cytokinin nucleotides is essential for elucidation of early steps of the biosynthetic pathway. However, traditional procedures for purification and quantification of cytokinin cannot discriminate the various nucleotides. We describe here a method for separation and determination of cytokinin nucleotides through a series of anion-exchange column chromatography steps followed by liquid chromatography-mass spectrometry. This method enabled us to analyze the amount of each species of cytokinin nucleotide in plant tissues.

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Year:  2003        PMID： 12734720     DOI： 10.1007/s10265-003-0099-1

Source DB:  PubMed          Journal:  J Plant Res        ISSN： 0918-9440            Impact factor:   2.629


  14 in total

1.  An alternative cytokinin biosynthesis pathway.

Authors:  C Astot; K Dolezal; A Nordström; Q Wang; T Kunkel; T Moritz; N H Chua; G Sandberg
Journal:  Proc Natl Acad Sci U S A       Date:  2000-12-19       Impact factor: 11.205

2.  CYTOKININ METABOLISM AND ACTION.

Authors:  David WS Mok; Machteld C Mok
Journal:  Annu Rev Plant Physiol Plant Mol Biol       Date:  2001-06

3.  Modification of cytokinins by cauliflower microsomal enzymes.

Authors:  C M Chen; S M Leisner
Journal:  Plant Physiol       Date:  1984-06       Impact factor: 8.340

4.  Nitrogen-dependent accumulation of cytokinins in root and the translocation to leaf: implication of cytokinin species that induces gene expression of maize response regulator.

Authors:  K Takei; H Sakakibara; M Taniguchi; T Sugiyama
Journal:  Plant Cell Physiol       Date:  2001-01       Impact factor: 4.927

5.  Identification of plant cytokinin biosynthetic enzymes as dimethylallyl diphosphate:ATP/ADP isopentenyltransferases.

Authors:  T Kakimoto
Journal:  Plant Cell Physiol       Date:  2001-07       Impact factor: 4.927

6.  Identification of genes encoding adenylate isopentenyltransferase, a cytokinin biosynthesis enzyme, in Arabidopsis thaliana.

Authors:  K Takei; H Sakakibara; T Sugiyama
Journal:  J Biol Chem       Date:  2001-04-19       Impact factor: 5.157

7.  Effects of sulfur nutrition on expression of the soybean seed storage protein genes in transgenic petunia.

Authors:  T Fujiwara; M Y Hirai; M Chino; Y Komeda; S Naito
Journal:  Plant Physiol       Date:  1992-05       Impact factor: 8.340

8.  Identification of Cytokinin Biosynthesis Genes in Arabidopsis: A Breakthrough for Understanding the Metabolic Pathway and the Regulation in Higher Plants.

Authors:  Hitoshi Sakakibara; Kentaro Takei
Journal:  J Plant Growth Regul       Date:  2002-03       Impact factor: 4.169

9.  A new method for enzymatic preparation of isopentenyladenine-type and trans-zeatin-type cytokinins with radioisotope-labeling.

Authors:  Kentaro Takei; Yasumasa Dekishima; Tadashi Eguchi; Tomoyuki Yamaya; Hitoshi Sakakibara
Journal:  J Plant Res       Date:  2003-05-01       Impact factor: 2.629

10.  T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis.

Authors:  D E Akiyoshi; H Klee; R M Amasino; E W Nester; M P Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205
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  2 in total

1.  Highly sensitive and high-throughput analysis of plant hormones using MS-probe modification and liquid chromatography-tandem mass spectrometry: an application for hormone profiling in Oryza sativa.

Authors:  Mikiko Kojima; Tomoe Kamada-Nobusada; Hirokazu Komatsu; Kentaro Takei; Takeshi Kuroha; Masaharu Mizutani; Motoyuki Ashikari; Miyako Ueguchi-Tanaka; Makoto Matsuoka; Koji Suzuki; Hitoshi Sakakibara
Journal:  Plant Cell Physiol       Date:  2009-04-15       Impact factor: 4.927

2.  A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction.

Authors:  Jana Svačinová; Ondřej Novák; Lenka Plačková; René Lenobel; Josef Holík; Miroslav Strnad; Karel Doležal
Journal:  Plant Methods       Date:  2012-05-17       Impact factor: 4.993
  2 in total

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