| Literature DB >> 11949214 |
Dirk C De Graaf1, Hans De Coninck, Franz Petry, Ilka B Eeckhout, Johan E Peeters.
Abstract
A Cryptosporidium parvum sporozoite and oocyst lambda gt11 cDNA library was screened with a hyperimmune rabbit serum that was developed against insoluble fragments of ultrasonicated oocysts. A clone named Cp22.4.1 encoding a protein of 231 amino acids with 4 zinc-finger domains characterized by a Cys-X2-Cys-X4-His-X4-Cys motif was isolated and characterized. There was a complete match between the sequencing data of the coding region of Cp22.4.1 and the corresponding gene at chromosomal level. Cloning in a pBAD-TOPO-TA expression vector permitted to evaluate the antigenicity of the recombinant His-tagged antigen. This antigen was recognized by 2 out of 5 sera from Cryptosporidium immune calves and not by sera from parasite naive animals.Entities:
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Year: 2002 PMID： 11949214 PMCID： PMC2721057 DOI： 10.3347/kjp.2002.40.1.59
Source DB: PubMed Journal: Korean J Parasitol ISSN： 0023-4001 Impact factor: 1.341