Literature DB >> 11948174

Characterization of plasmid pRT1 from Pyrococcus sp. strain JT1.

Donald E Ward1, Ingrid M Revet, Renu Nandakumar, Jon H Tuttle, Willem M de Vos, John van der Oost, Jocelyne DiRuggiero.   

Abstract

We discovered a 3,373-bp plasmid (pRT1) in the hyperthermophilic archaeon Pyrococcus sp. strain JT1. Two major open reading frames were identified, and analysis of the sequence revealed some resemblance to motifs typically found in plasmids that replicate via a rolling-circle mechanism. The presence of single-stranded DNA replication intermediates of pRT1 was detected, confirming this mode of replication.

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Year:  2002        PMID: 11948174      PMCID: PMC134983          DOI: 10.1128/JB.184.9.2561-2566.2002

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


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