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Literature DB >> 1194267

Function of 5'-nucleotidase in the uptake of adenosine from AMP by human lymphocytes.

H Fleit, M Conklyn, R D Stebbins, R Silber.

Abstract

The function of 5'-nucleotidase in nucleoside uptake from AMP was investigated in human lymphocytes by comparing the transport in cells containing this enzyme (5'N+) with that in cells deficient in the activity (5'N-). The rate of adenosine and Pi uptake from AMP was 3.9-fold greater in the 5'N+ then in the 5'N- lymphocytes. There was no difference in transport between these cells when incubated with adenosine or Pi. These results indicate that phosphorylytic cleavage of AMP by 5'-nucleotidase is necessary for the uptake of the nucleotide and Pi moieties by the human lymphocyte.

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Year: 1975 PMID： 1194267

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157

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Cited

15 in total

1. Enzyme activities in endothelial cells and smooth muscle cells from swine aorta.

Authors: L W Hayes; C A Goguen; A L Stevens; W W Magargal; L L Slakey
Journal: Proc Natl Acad Sci U S A Date: 1979-06 Impact factor: 11.205

2. Enzymes involved in adenosine metabolism in rat white and brown adipocytes. Effects of streptozotocin-diabetes, hypothyroidism, age and sex differences.

Authors: Z Jamal; E D Saggerson
Journal: Biochem J Date: 1987-08-01 Impact factor: 3.857

3. Uptake of free adenosine and adenosine from adenosine monophosphate by human peripheral blood lymphocytes: possible kinetic role for ecto-5'-nucleotidase in the regulation of intracellular adenosine.

Authors: T Shah; R J Simpson; A D Webster; T J Peters
Journal: Clin Exp Immunol Date: 1986-10 Impact factor: 4.330

8. Correlations between enzymatic and immunologic properties of human peripheral blood mononuclear cells. I. Ectoenzymes of normal and immunodeficient peripheral blood mononuclear cells.

Authors: G Losa; A Morell; S Barandun
Journal: Am J Pathol Date: 1982-05 Impact factor: 4.307

9. 5'-Nucleotidase activities in sera and liver tissues of viral hepatitis patients.

Authors: M Fukano; S Amano; F Hazama; S Hosoda
Journal: Gastroenterol Jpn Date: 1990-04

10. Transport of 9-(2-phosphonomethoxyethyl)adenine across plasma membrane of HeLa S3 cells is protein mediated.

Authors: T Cihlár; I Rosenberg; I Votruba; A Holý
Journal: Antimicrob Agents Chemother Date: 1995-01 Impact factor: 5.191

Function of 5'-nucleotidase in the uptake of adenosine from AMP by human lymphocytes.

1. Enzyme activities in endothelial cells and smooth muscle cells from swine aorta.

2. Enzymes involved in adenosine metabolism in rat white and brown adipocytes. Effects of streptozotocin-diabetes, hypothyroidism, age and sex differences.

3. Uptake of free adenosine and adenosine from adenosine monophosphate by human peripheral blood lymphocytes: possible kinetic role for ecto-5'-nucleotidase in the regulation of intracellular adenosine.

4. Inosine 5'-phosphate dehydrogenase activity in normal and leukemic blood cells.

5. Sodium gradient-energized concentrative transport of adenosine in renal brush border vesicles.

6. Adenosine uptake in pyrimidine 5'-nucleotidase deficient human erythrocytes via a high affinity transport system.

7. A kinetic study of the soluble 5'-nucleotidase of rat liver.

8. Correlations between enzymatic and immunologic properties of human peripheral blood mononuclear cells. I. Ectoenzymes of normal and immunodeficient peripheral blood mononuclear cells.

9. 5'-Nucleotidase activities in sera and liver tissues of viral hepatitis patients.

10. Transport of 9-(2-phosphonomethoxyethyl)adenine across plasma membrane of HeLa S3 cells is protein mediated.