BACKGROUND: Dust reservoir sampling is the most commonly used method for assessment of indirect allergen exposure. Because assessment of personal exposure using person-carried pumps is time-consuming and expensive we evaluated the Petri dish sampling method for measurement of airborne cat allergen in classrooms. METHODS: Petri dish sampling was evaluated in three study parts. Part I: by comparison between Petri dish sampling and personal air sampling in 44 classrooms with many (> or = 20%) and few (< or = 10%) cat owners and by additional Petri dish sampling in 40 pet-free homes. Part II: by sampling with duplicate Petri dishes in 28 classrooms. Part III: by sampling in three classrooms at four sampling heights during different sampling times. All samples were analyzed for cat allergen (Fel d 1) content with a monoclonal antibody two-site ELISA (enzyme linked immunosorbent assay), using signal amplification when necessary. RESULTS: There was a significant correlation between Petri dish sampling and personal air sampling (r = 0.66; P < 0.0001). Levels were five-fold higher in classes with many cat owners than in classes with few cat owners, regardless of method. A corresponding difference was found in the homes. Duplicate sample values were in fair agreement (Bland-Altman test) and were correlated (r = 0.77; P < 0.0001). Cumulative levels collected weekly in one Petri dish were lower than using five daily Petri dishes, regardless of sampling height. CONCLUSIONS: Petri dish sampling can be useful as an alternative method to personal air sampling of airborne allergens.
BACKGROUND: Dust reservoir sampling is the most commonly used method for assessment of indirect allergen exposure. Because assessment of personal exposure using person-carried pumps is time-consuming and expensive we evaluated the Petri dish sampling method for measurement of airborne cat allergen in classrooms. METHODS: Petri dish sampling was evaluated in three study parts. Part I: by comparison between Petri dish sampling and personal air sampling in 44 classrooms with many (> or = 20%) and few (< or = 10%) cat owners and by additional Petri dish sampling in 40 pet-free homes. Part II: by sampling with duplicate Petri dishes in 28 classrooms. Part III: by sampling in three classrooms at four sampling heights during different sampling times. All samples were analyzed for cat allergen (Fel d 1) content with a monoclonal antibody two-site ELISA (enzyme linked immunosorbent assay), using signal amplification when necessary. RESULTS: There was a significant correlation between Petri dish sampling and personal air sampling (r = 0.66; P < 0.0001). Levels were five-fold higher in classes with many cat owners than in classes with few cat owners, regardless of method. A corresponding difference was found in the homes. Duplicate sample values were in fair agreement (Bland-Altman test) and were correlated (r = 0.77; P < 0.0001). Cumulative levels collected weekly in one Petri dish were lower than using five daily Petri dishes, regardless of sampling height. CONCLUSIONS: Petri dish sampling can be useful as an alternative method to personal air sampling of airborne allergens.
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