| Literature DB >> 11929231 |
Chun-Cheng Lin1, Yi-Chun Yeh, Chan-Yi Yang, Chan-Long Chen, Gee-Fong Chen, Chia-Chun Chen, Yi-Chun Wu.
Abstract
The synthesis, characterization and biological application of mannose encapsulated gold nanoparticles (m-AuNP) are reported. m-AuNP is well dispersed and very stable without aggregation in the media of broad ion strength and pH ranges. The selective binding of m-AuNP to the mannose adhesin FimH of bacterial type 1 pili is demonstrated using transmission electron microscopy. The competition assay with free mannose suggests that m-AuNP binds FimH better than free mannose does. This work demonstrates that carbohydrate attached nanoparticles can be used as an efficient affinity label and a multi-ligand carrier in a biological system.Entities:
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Year: 2002 PMID： 11929231 DOI： 10.1021/ja0200903
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419