AIM: To investigate the relationship among gastrin, somatostatin, G and D cells in gastric ulcer and in its healing process in rats. METHODS: Fourty-nine Wistar rats were divided into 7 groups. The gastric ulcer model was induced by acetic acid successfully. The gastrin and the somatostatin in rat plasma, gastric fluid and antral tissue were measured by radioimmunoassay(RIA). G and D cells in antral mucosa were analyzed with polyclonal antibody of gastrin and somatostatin by immunohistochemical method and Quantimet 500 image analysis system. RESULTS: In gastric ulcer, the level of gastrin in plasma, gastric fluid, and antral tissue increased, that of somatostatin declined, and the disorder gradually recovered to the normal level in the healing process. Immunohistochemical technique of G and D cells in antral mucosa demonstrated that the number of G cells increased and that of D cells decreased, both areas of G and D cells declined, the ratio of number and area of G/D increased in gastric ulcer, and the disorder gradually recovered in the healing process. CONCLUSION: In gastric ulcer, the increased gastrin secreted by G cells, the declined somatostatin secreted by D cells, and the disordered G/D cell ratio can lead to gastrointestinal dysfunction.
AIM: To investigate the relationship among gastrin, somatostatin, G and D cells in gastric ulcer and in its healing process in rats. METHODS: Fourty-nine Wistar rats were divided into 7 groups. The gastric ulcer model was induced by acetic acid successfully. The gastrin and the somatostatin in rat plasma, gastric fluid and antral tissue were measured by radioimmunoassay(RIA). G and D cells in antral mucosa were analyzed with polyclonal antibody of gastrin and somatostatin by immunohistochemical method and Quantimet 500 image analysis system. RESULTS: In gastric ulcer, the level of gastrin in plasma, gastric fluid, and antral tissue increased, that of somatostatin declined, and the disorder gradually recovered to the normal level in the healing process. Immunohistochemical technique of G and D cells in antral mucosa demonstrated that the number of G cells increased and that of D cells decreased, both areas of G and D cells declined, the ratio of number and area of G/D increased in gastric ulcer, and the disorder gradually recovered in the healing process. CONCLUSION: In gastric ulcer, the increased gastrin secreted by G cells, the declined somatostatin secreted by D cells, and the disordered G/D cell ratio can lead to gastrointestinal dysfunction.
Authors: T Iyo; H Kaneko; T Konagaya; S Mori; H Kotera; M Uruma; N Rhue; T Shimizu; A Imada; K Kusugami; T Mitsuma Journal: Life Sci Date: 1999 Impact factor: 5.037
Authors: S Hiraoka; Y Miyazaki; S Kitamura; M Toyota; T Kiyohara; Y Shinomura; N Mukaida; Y Matsuzawa Journal: Am J Physiol Gastrointest Liver Physiol Date: 2001-09 Impact factor: 4.052
Authors: Leônia Maria Batista; Gedson Rodrigues De Morais Lima; Ana Beatriz Albino De Almeida; Luciana De Pietro Magri; Tamara Regina Calvo; Anderson Luiz Ferreira; Cláudia Helena Pellizzon; Clélia Akiko Hiruma-Lima; Wagner Vilegas; Paulo Takeo Sano; Alba Regina Monteiro Souza Brito Journal: BMC Complement Altern Med Date: 2015-10-29 Impact factor: 3.659