Feng-Peng Sun1, Yu-Gang Song. 1. Department of Gastroenterology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, Guangdong Province, China. sci@china.com
Abstract
AIM: To investigate the gastrin secreting cells (G cells) and the somatostatin secreting cells (D cells) of antral mucosa in rats at the ultrastructural level. METHODS: Revised immunoelectron microscopic technique was used to detect the G cells and D cells in rat antral mucosa through gastrin and somatostatin antibodies labeled by colloidal gold. Also the relevant quantitative analysis regarding the granular number of colloidal gold in G cells and in D cells was conducted. RESULTS: Immunological granules of colloidal gold were distributed in G cells and D cells. Gastrin labeled golden granules or somatostatin labeled ones presented mainly as lobation-like or island-like congeries. Most of the golden congeries were observed dissociated in cytoplasms of G cells or D cells, near the basement membrane. A few golden congeries were located in nuclei. The number of golden granules in one G cell was around 107.04 +/- 19.68 and was 83.36 +/- 17.58 in one D cell. CONCLUSION: Gastrin secreting granules are located in cytoplasms and nuclei of G cells, and somatostatin secreting granules both in cytoplasms and in nuclei of D cells. The number of golden granules can be quantitatively analyzed to determine the relative amount of gastrin secreting granules or somatostatin secreting granules.
AIM: To investigate the gastrin secreting cells (G cells) and the somatostatin secreting cells (D cells) of antral mucosa in rats at the ultrastructural level. METHODS: Revised immunoelectron microscopic technique was used to detect the G cells and D cells in rat antral mucosa through gastrin and somatostatin antibodies labeled by colloidal gold. Also the relevant quantitative analysis regarding the granular number of colloidal gold in G cells and in D cells was conducted. RESULTS: Immunological granules of colloidal gold were distributed in G cells and D cells. Gastrin labeled golden granules or somatostatin labeled ones presented mainly as lobation-like or island-like congeries. Most of the golden congeries were observed dissociated in cytoplasms of G cells or D cells, near the basement membrane. A few golden congeries were located in nuclei. The number of golden granules in one G cell was around 107.04 +/- 19.68 and was 83.36 +/- 17.58 in one D cell. CONCLUSION:Gastrin secreting granules are located in cytoplasms and nuclei of G cells, and somatostatin secreting granules both in cytoplasms and in nuclei of D cells. The number of golden granules can be quantitatively analyzed to determine the relative amount of gastrin secreting granules or somatostatin secreting granules.
Authors: M E Caplin; P Clarke; S Grimes; A P Dhillon; K Khan; K Savage; J Lewin; D Michaeli; R E Pounder; S A Watson Journal: Regul Pept Date: 1999-10-22
Authors: S Ishida; M Kaito; M Kohara; K Tsukiyama-Kohora; N Fujita; J Ikoma; Y Adachi; S Watanabe Journal: Hepatol Res Date: 2001-07 Impact factor: 4.288
Authors: T Iyo; H Kaneko; T Konagaya; S Mori; H Kotera; M Uruma; N Rhue; T Shimizu; A Imada; K Kusugami; T Mitsuma Journal: Life Sci Date: 1999 Impact factor: 5.037
Authors: M Takahashi; Y Hoshii; H Kawano; M Setoguchi; T Gondo; Y Yamashita; K Nakayasu; T Kamei; T Ishihara Journal: Am J Surg Pathol Date: 1998-03 Impact factor: 6.394