BACKGROUND: Concentrations of certain substances in follicular fluid (FF) are related to fertilization outcome and early post-fertilization development. The study aim was to identify FF markers with which to predict embryo implantation potential. METHODS: Concentrations of selected hormones, cytokines and growth factors in individual FF samples obtained during assisted reproduction treatment were related with treatment outcomes. RESULTS: Mean concentrations of LH, growth hormone (GH), prolactin (PRL), 17beta-estradiol (E2) and insulin-like growth factor (IGF)-I were higher, and that of interleukin-1 (IL-1) was lower, in treatment attempts leading to a clinical pregnancy as compared with those in which no pregnancy was established. Concentrations of FSH, progesterone, tumour necrosis factor-alpha and IGF-II were similar in successful and unsuccessful attempts. In successful attempts, LH and GH levels were higher in those follicles from which oocytes giving rise to transferred embryos (i.e. embryos with best morphology and fastest cleavage rate) originated, as compared with other follicles from which a mature oocyte was recovered but was cryopreserved for later use. CONCLUSIONS: FF levels of LH, GH, PRL, E2, IGF-I and IL-1 may serve to analyse cases of repeated assisted reproduction failures and to assess effects of modifications of the ovarian stimulation protocol.
BACKGROUND: Concentrations of certain substances in follicular fluid (FF) are related to fertilization outcome and early post-fertilization development. The study aim was to identify FF markers with which to predict embryo implantation potential. METHODS: Concentrations of selected hormones, cytokines and growth factors in individual FF samples obtained during assisted reproduction treatment were related with treatment outcomes. RESULTS: Mean concentrations of LH, growth hormone (GH), prolactin (PRL), 17beta-estradiol (E2) and insulin-like growth factor (IGF)-I were higher, and that of interleukin-1 (IL-1) was lower, in treatment attempts leading to a clinical pregnancy as compared with those in which no pregnancy was established. Concentrations of FSH, progesterone, tumour necrosis factor-alpha and IGF-II were similar in successful and unsuccessful attempts. In successful attempts, LH and GH levels were higher in those follicles from which oocytes giving rise to transferred embryos (i.e. embryos with best morphology and fastest cleavage rate) originated, as compared with other follicles from which a mature oocyte was recovered but was cryopreserved for later use. CONCLUSIONS: FF levels of LH, GH, PRL, E2, IGF-I and IL-1 may serve to analyse cases of repeated assisted reproduction failures and to assess effects of modifications of the ovarian stimulation protocol.
Authors: Martin Wilding; Michael Singer; Peter Fehr; Felix Haeberlin; Felix Roth; Remo Lachat; Loredana Di Matteo; Clemente Capobianco; Brian Dale Journal: J Assist Reprod Genet Date: 2006-04-19 Impact factor: 3.412
Authors: G De Placido; C Alviggi; R Clarizia; A Mollo; E Alviggi; I Strina; E Fiore; M Wilding; T Pagano; G Matarese Journal: J Endocrinol Invest Date: 2006-09 Impact factor: 4.256
Authors: Irma Virant-Klun; S Omejec; M Stimpfel; P Skerl; S Novakovic; N Jancar; E Vrtacnik-Bokal Journal: Stem Cell Rev Rep Date: 2019-08 Impact factor: 5.739
Authors: Y Ganor-Paz; Y Friedler-Mashiach; Y Ghetler; A Hershko-Klement; A Berkovitz; O Gonen; A Shulman; A Wiser Journal: J Endocrinol Invest Date: 2016-01-21 Impact factor: 4.256
Authors: Mete Ahmet Ergenoğlu; Ahmet Özgür Yeniel; Ayşin Akdoğan; Ege Nazan Tavmergen Göker; Erol Tavmergen Journal: J Turk Ger Gynecol Assoc Date: 2012-06-01