Literature DB >> 11917039

TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp., recognizes novel asymmetric sequence 5'-ACGGA(N11/9)-3'.

Agnieszka Zylicz-Stachula1, Renata I Harasimowicz-Slowińska, Ireneusz Sobolewski, Piotr M Skowron.   

Abstract

A novel prototype class-IIS restriction endonuclease, TspGWI, was isolated from the thermophilic bacterium Thermus sp. GW. The recognition sequence and cleavage positions have been established: TspGWI recognizes the non-palindromic 5-bp sequence 5'-ACGGA-3' and cleaves the DNA 11 and 9 nt downstream in the top and bottom strand, respectively. In addition, an accompanying endonuclease, TspGWII, an isoschizomer of Pst I, was found in Thermus sp. GW cells.

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Year:  2002        PMID： 11917039      PMCID： PMC101857          DOI： 10.1093/nar/30.7.e33

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  5 in total

1.  REBASE--restriction enzymes and methylases.

Authors:  R J Roberts; D Macelis
Journal:  Nucleic Acids Res       Date:  2001-01-01       Impact factor: 16.971

Review 2.  Class-IIS restriction enzymes--a review.

Authors:  W Szybalski; S C Kim; N Hasan; A J Podhajska
Journal:  Gene       Date:  1991-04       Impact factor: 3.688

3.  A second type II restriction endonuclease from Thermus aquaticus with an unusual sequence specificity.

Authors:  D Barker; M Hoff; A Oliphant; R White
Journal:  Nucleic Acids Res       Date:  1984-07-25       Impact factor: 16.971

4.  A second site specific endonuclease from Thermus thermophilus 111, Tth111II.

Authors:  T Shinomiya; M Kobayashi; S Sato
Journal:  Nucleic Acids Res       Date:  1980-08-11       Impact factor: 16.971

5.  Single-stranded DNA 'blue' T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering.

Authors:  D A Mead; E Szczesna-Skorupa; B Kemper
Journal:  Protein Eng       Date:  1986 Oct-Nov
  5 in total
  13 in total

1.  A new Thermus sp. class-IIS enzyme sub-family: isolation of a 'twin' endonuclease TspDTI with a novel specificity 5'-ATGAA(N(11/9))-3', related to TspGWI, TaqII and Tth111II.

Authors:  Piotr M Skowron; Jarosław Majewski; Agnieszka Zylicz-Stachula; Sylwia M Rutkowska; Izabela Jaworowska; Renata I Harasimowicz-Słowińska
Journal:  Nucleic Acids Res       Date:  2003-07-15       Impact factor: 16.971

2.  Engineering TaqII bifunctional endonuclease DNA recognition fidelity: the effect of a single amino acid substitution within the methyltransferase catalytic site.

Authors:  Agnieszka Zylicz-Stachula; Joanna Zebrowska; Edyta Czajkowska; Weronika Wrese; Ewa Sulecka; Piotr M Skowron
Journal:  Mol Biol Rep       Date:  2016-02-17       Impact factor: 2.316

3.  A putative Type IIS restriction endonuclease GeoICI from Geobacillus sp.--A robust, thermostable alternative to mezophilic prototype BbvI.

Authors:  Joanna Zebrowska; Olga Zolnierkiewicz; Marta A Skowron; Agnieszka Zylicz-Stachula; Joanna Jezewska-Frackowiak; Piotr M Skowron
Journal:  J Biosci       Date:  2016-03       Impact factor: 1.826

4.  Bifunctional TaqII restriction endonuclease: redefining the prototype DNA recognition site and establishing the Fidelity Index for partial cleaving.

Authors:  Agnieszka Zylicz-Stachula; Olga Zołnierkiewicz; Katarzyna Sliwińska; Joanna Jeżewska-Frąckowiak; Piotr M Skowron
Journal:  BMC Biochem       Date:  2011-12-05       Impact factor: 4.059

5.  Related bifunctional restriction endonuclease-methyltransferase triplets: TspDTI, Tth111II/TthHB27I and TsoI with distinct specificities.

Authors:  Agnieszka Zylicz-Stachula; Olga Zolnierkiewicz; Arvydas Lubys; Danute Ramanauskaite; Goda Mitkaite; Janusz M Bujnicki; Piotr M Skowron
Journal:  BMC Mol Biol       Date:  2012-04-10       Impact factor: 2.946

6.  Characterization of cleavage intermediate and star sites of RM.Tth111II.

Authors:  Zhenyu Zhu; Shengxi Guan; Derek Robinson; Hanna El Fezzazi; Aine Quimby; Shuang-yong Xu
Journal:  Sci Rep       Date:  2014-01-23       Impact factor: 4.379

7.  Modified 'one amino acid-one codon' engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase.

Authors:  Agnieszka Zylicz-Stachula; Olga Zolnierkiewicz; Katarzyna Sliwinska; Joanna Jezewska-Frackowiak; Piotr M Skowron
Journal:  Microb Cell Fact       Date:  2014-01-11       Impact factor: 5.328

8.  Cloning and analysis of a bifunctional methyltransferase/restriction endonuclease TspGWI, the prototype of a Thermus sp. enzyme family.

Authors:  Agnieszka Zylicz-Stachula; Janusz M Bujnicki; Piotr M Skowron
Journal:  BMC Mol Biol       Date:  2009-05-29       Impact factor: 2.946

9.  A new genomic tool, ultra-frequently cleaving TaqII/sinefungin endonuclease with a combined 2.9-bp recognition site, applied to the construction of horse DNA libraries.

Authors:  Agnieszka Zylicz-Stachula; Olga Zolnierkiewicz; Jacek Jasiecki; Piotr M Skowron
Journal:  BMC Genomics       Date:  2013-06-01       Impact factor: 3.969

10.  Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus.

Authors:  Piotr M Skowron; Jolanta Vitkute; Danute Ramanauskaite; Goda Mitkaite; Joanna Jezewska-Frackowiak; Joanna Zebrowska; Agnieszka Zylicz-Stachula; Arvydas Lubys
Journal:  BMC Mol Biol       Date:  2013-08-06       Impact factor: 2.946
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