Literature DB >> 11917035

An in vivo selection system for homing endonuclease activity.

Mathias Gruen1, Kathy Chang, Irina Serbanescu, David R Liu.   

Abstract

Homing endonucleases are enzymes that catalyze the highly sequence-specific cleavage of DNA. We have developed an in vivo selection in Escherichia coli that links cell survival with homing endonuclease-mediated DNA cleavage activity and sequence specificity. Using this selection, wild-type and mutant variants of three homing endonucleases were characterized without requiring protein purification and in vitro analysis. This selection system may facilitate the study of sequence-specific DNA cleaving enzymes, and selections based on this work may enable the evolution of homing endonucleases with novel activities or specificities.

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Year:  2002        PMID： 11917035      PMCID： PMC101853          DOI： 10.1093/nar/30.7.e29

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  27 in total

1.  Chemical probing shows that the intron-encoded endonuclease I-SceI distorts DNA through binding in monomeric form to its homing site.

Authors:  B Beylot; A Spassky
Journal:  J Biol Chem       Date:  2001-03-12       Impact factor: 5.157

Review 2.  Homing endonucleases: structure, function and evolution.

Authors:  M S Jurica; B L Stoddard
Journal:  Cell Mol Life Sci       Date:  1999-08-15       Impact factor: 9.261

3.  Using molecular beacons as a sensitive fluorescence assay for enzymatic cleavage of single-stranded DNA.

Authors:  J J Li; R Geyer; W Tan
Journal:  Nucleic Acids Res       Date:  2000-06-01       Impact factor: 16.971

Review 4.  Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility.

Authors:  B S Chevalier; B L Stoddard
Journal:  Nucleic Acids Res       Date:  2001-09-15       Impact factor: 16.971

5.  Barnase and barstar. Expression of its cloned inhibitor permits expression of a cloned ribonuclease.

Authors:  R W Hartley
Journal:  J Mol Biol       Date:  1988-08-20       Impact factor: 5.469

6.  Recognition and cleavage site of the intron-encoded omega transposase.

Authors:  L Colleaux; L D'Auriol; F Galibert; B Dujon
Journal:  Proc Natl Acad Sci U S A       Date:  1988-08       Impact factor: 11.205

7.  Effects of functional group changes in the EcoRI recognition site on the cleavage reaction catalyzed by the endonuclease.

Authors:  L W McLaughlin; F Benseler; E Graeser; N Piel; S Scholtissek
Journal:  Biochemistry       Date:  1987-11-17       Impact factor: 3.162

Review 8.  Barnase and barstar: two small proteins to fold and fit together.

Authors:  R W Hartley
Journal:  Trends Biochem Sci       Date:  1989-11       Impact factor: 13.807

9.  Critical base substitutions that affect the splicing and/or homing activities of the group I intron bi2 of yeast mitochondria.

Authors:  T Szczepanek; K Jamoussi; J Lazowska
Journal:  Mol Gen Genet       Date:  2000-09

10.  The monomeric homing endonuclease PI-SceI has two catalytic centres for cleavage of the two strands of its DNA substrate.

Authors:  F Christ; S Schoettler; W Wende; S Steuer; A Pingoud; V Pingoud
Journal:  EMBO J       Date:  1999-12-15       Impact factor: 11.598
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  13 in total

1.  In vitro selection of restriction endonucleases by in vitro compartmentalization.

Authors:  Nobuhide Doi; Shin Kumadaki; Yuko Oishi; Nobutaka Matsumura; Hiroshi Yanagawa
Journal:  Nucleic Acids Res       Date:  2004-07-06       Impact factor: 16.971

2.  Microhomology-mediated and nonhomologous repair of a double-strand break in the chloroplast genome of Arabidopsis.

Authors:  Taegun Kwon; Enamul Huq; David L Herrin
Journal:  Proc Natl Acad Sci U S A       Date:  2010-07-19       Impact factor: 11.205

3.  Directed evolution of homing endonuclease I-SceI with altered sequence specificity.

Authors:  Zhilei Chen; Fei Wen; Ning Sun; Huimin Zhao
Journal:  Protein Eng Des Sel       Date:  2009-01-28       Impact factor: 1.650

Review 4.  Homing endonucleases: from microbial genetic invaders to reagents for targeted DNA modification.

Authors:  Barry L Stoddard
Journal:  Structure       Date:  2011-01-12       Impact factor: 5.006

Review 5.  Homing endonucleases: from genetic anomalies to programmable genomic clippers.

Authors:  Marlene Belfort; Richard P Bonocora
Journal:  Methods Mol Biol       Date:  2014

Review 6.  Homing endonucleases: from basics to therapeutic applications.

Authors:  Maria J Marcaida; Inés G Muñoz; Francisco J Blanco; Jesús Prieto; Guillermo Montoya
Journal:  Cell Mol Life Sci       Date:  2010-03       Impact factor: 9.261

7.  Creating highly specific nucleases by fusion of active restriction endonucleases and catalytically inactive homing endonucleases.

Authors:  Ines Fonfara; Ute Curth; Alfred Pingoud; Wolfgang Wende
Journal:  Nucleic Acids Res       Date:  2011-09-29       Impact factor: 16.971

8.  A highly sensitive selection method for directed evolution of homing endonucleases.

Authors:  Zhilei Chen; Huimin Zhao
Journal:  Nucleic Acids Res       Date:  2005-10-06       Impact factor: 16.971

9.  In vivo selection of engineered homing endonucleases using double-strand break induced homologous recombination.

Authors:  Patrick Chames; Jean-Charles Epinat; Sophie Guillier; Amélie Patin; Emmanuel Lacroix; Frédéric Pâques
Journal:  Nucleic Acids Res       Date:  2005-11-23       Impact factor: 16.971

10.  Thermodynamics of DNA target site recognition by homing endonucleases.

Authors:  Jennifer H Eastberg; Audrey McConnell Smith; Lei Zhao; Justin Ashworth; Betty W Shen; Barry L Stoddard
Journal:  Nucleic Acids Res       Date:  2007-10-18       Impact factor: 16.971
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