Literature DB >> 11914505

Purification and crystallization of the yeast elongation factor eEF2.

René Jørgensen1, Anne Carr-Schmid, Pedro A Ortiz, Terri Goss Kinzy, Gregers Rom Andersen.   

Abstract

Crystals of the Saccharomyces cerevisiae elongation factor 2 (eEF2) in complex with GDP were obtained with the vapour-diffusion technique after rapid purification from industrial yeast. The crystals diffract to 2.85 A and belong to the space group P2(1)2(1)2(1). A yeast strain expressing a functional histidine-tagged eEF2 as the only form of the protein further allows facilitated purification of the factor for both structural and functional studies.

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Year:  2002        PMID： 11914505     DOI： 10.1107/s0907444902003001

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  20 in total

1.  Multiple Parallel Pathways of Translation Initiation on the CrPV IRES.

Authors:  Alexey Petrov; Rosslyn Grosely; Jin Chen; Seán E O'Leary; Joseph D Puglisi
Journal:  Mol Cell       Date:  2016-04-07       Impact factor: 17.970

2.  A conserved eEF2 coding variant in SCA26 leads to loss of translational fidelity and increased susceptibility to proteostatic insult.

Authors:  Katherine E Hekman; Guo-Yun Yu; Christopher D Brown; Haipeng Zhu; Xiaofei Du; Kristina Gervin; Dag Erik Undlien; April Peterson; Giovanni Stevanin; H Brent Clark; Stefan M Pulst; Thomas D Bird; Kevin P White; Christopher M Gomez
Journal:  Hum Mol Genet       Date:  2012-09-21       Impact factor: 6.150

3.  Identification and characterization of a novel evolutionarily conserved lysine-specific methyltransferase targeting eukaryotic translation elongation factor 2 (eEF2).

Authors:  Erna Davydova; Angela Y Y Ho; Jedrzej Malecki; Anders Moen; Jorrit M Enserink; Magnus E Jakobsson; Christoph Loenarz; Pål Ø Falnes
Journal:  J Biol Chem       Date:  2014-09-17       Impact factor: 5.157

4.  Distinct response of yeast ribosomes to a miscoding event during translation.

Authors:  Daniel E Eyler; Rachel Green
Journal:  RNA       Date:  2011-03-17       Impact factor: 4.942

5.  ADP-ribosylation of translation elongation factor 2 by diphtheria toxin in yeast inhibits translation and cell separation.

Authors:  Maria K Mateyak; Terri Goss Kinzy
Journal:  J Biol Chem       Date:  2013-07-12       Impact factor: 5.157

6.  eIF5A Functions Globally in Translation Elongation and Termination.

Authors:  Anthony P Schuller; Colin Chih-Chien Wu; Thomas E Dever; Allen R Buskirk; Rachel Green
Journal:  Mol Cell       Date:  2017-04-06       Impact factor: 17.970

7.  Structure of the C-Terminal Helical Repeat Domain of Eukaryotic Elongation Factor 2 Kinase.

Authors:  Nathan Will; Andrea Piserchio; Isaac Snyder; Scarlet B Ferguson; David H Giles; Kevin N Dalby; Ranajeet Ghose
Journal:  Biochemistry       Date:  2016-09-14       Impact factor: 3.162

8.  Translation elongation factor 1A mutants with altered actin bundling activity show reduced aminoacyl-tRNA binding and alter initiation via eIF2α phosphorylation.

Authors:  Winder B Perez; Terri Goss Kinzy
Journal:  J Biol Chem       Date:  2014-07-25       Impact factor: 5.157

9.  De Novo variants in EEF2 cause a neurodevelopmental disorder with benign external hydrocephalus.

Authors:  Maria J Nabais Sá; Alexandra N Olson; Grace Yoon; Graeme A M Nimmo; Christopher M Gomez; Michèl A Willemsen; Francisca Millan; Alexandra Schneider; Rolph Pfundt; Arjan P M de Brouwer; Jonathan D Dinman; Bert B A de Vries
Journal:  Hum Mol Genet       Date:  2021-02-25       Impact factor: 6.150

10.  The amidation step of diphthamide biosynthesis in yeast requires DPH6, a gene identified through mining the DPH1-DPH5 interaction network.

Authors:  Shanow Uthman; Christian Bär; Viktor Scheidt; Shihui Liu; Sara ten Have; Flaviano Giorgini; Michael J R Stark; Raffael Schaffrath
Journal:  PLoS Genet       Date:  2013-02-28       Impact factor: 5.917
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